- ￥300
- SIGMA
- 美国
- 11858882001-1盒
- 2026年04月22日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
瓶身
- 保质期：
coa
- 库存：
999
- 供应商：
麦飞生物
- 规格：
11858882001-1盒
Application
The High Pure Viral RNA Kit is designed to purify intact viral RNA from serum or plasma samples. Viral RNA is used for RT-PCR analysis directly after elution in PCR grade water.
RNA preparations obtained are suitable for RT-PCR; they are not tested for other applications.
Principle
How this product works
As a pre-requisite for the analysis of viral RNA by the reverse transcription polymerase chain reaction (RT-PCR), the isolation of the analyte from serum or plasma is required. The High Pure Viral RNA Kit accomplishes virus lysis by incubation of the sample in a special Binding Buffer. Subsequently nucleic acids bind specifically to the surface of glass fibers in the presence of a chaotropic salt (guanidine-HCl). The binding reaction occurs within seconds due to the disruption of the organized structure of water molecules and the interaction of nucleic acids with the glass fibers surface.
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文献和实验Viral RNA extraction-Molecular Biology
to extract viral RNA out from a formalin fixed, paraffin embended viral infected tissue. However after extraction, the ectracted RNA is either not pure (ratio 1.27-1.35) or RNA concentration too little (6 ug/ml).I run with a RT-PCR togather with positive
Viral Small RNA Cloning and Sequencing
At the current rate of technological progress, high-throughput sequencing of nucleic acids has become a commodity. These techniques are perfectly suitable for viral small RNAs sequencing and contribute to the understanding of many aspects
RT-LATE-PCR for mRNA and Viral RNA Detection and Quantification
) with one or more probes. Based on its properties, LATE-PCR is also useful for constructing multiplex assays. Viral RNA or RNA present in cells can be detected and quantified using LATE-PCR preceded by a reverse transcription (RT) step that converts RNA
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