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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见产品外包装
- 英文名:
Trehalase from porcine kidney buffered aqueous glycerol solution, >=1.0 units/mg protein
- 库存:
20
- 供应商:
上海吉至生化科技有限公司
- CAS号:
9025-52-9
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文献和实验Agarose Gel Electrophoresis of DNA
(also useful for lots of other information on molecular biology. Melt excised bands at 70°C cool and add equal volume of TE-buffered phenol and mix. Spin for 5 min, remove aqueous phase, repeat and EtOh ppt. the DNA. See also phenol-base method
GST Fusion Protein Preparation
oxidized.PMSF should be added just prior to lysis of bacteria,as it is unstable in aqueous solutions.Instead of PMSF try less-toxic AEBSF.Stock solution: 0.2M in H2O,use 1:100 nSec1,a sticky protein,gives a higher yield if 0.5M NaCl is added
Protocols for protein extraction from whole tissues for isoelectric focusing
. 2.Add 5 ml of extraction media (0.175 M Tris-HCl,pH 8.8,5% SDS,15% glycerol,0.3 M DTT)directly to mortar and continue grinding for an additional 30 sec. 3.Filter homogenate through two layers of miracloth into a 50 ml Falcon tube at room temperature
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