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- 2025年12月31日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
for future use below -18°C
- 保质期：
See instructions
- 英文名：
mACPP
- 库存：
常规产品有备货
- 供应商：
上海经科化学科技有限公司
- CAS号：
无
- 规格：
2ug/10ug/1mg
| 规格： | 2ug | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 10ug | 产品价格： | ￥2415.0 |
| 规格： | 1mg | 产品价格： | ￥87906.0 |
CATALOGUE NUMBER
ENZ-1157
SYNONYMS
acid phosphatase, prostate, ACP3, ACP-3, ACPP, EC 3.1.3.2, PAP, Prostatic Acid Phosphatase, prostatic acid phosphatase, 5-nucleotidase, 5'-NT, Acid phosphatase 3, Ecto-5'-nucleotidase, Fluoride-resistant acid phosphatase, FRAP, Thiamine monophosphatase, TMPase, A030005E02Rik, Lap, PAP, Ppal.
INTRODUCTION
Prostatic Acid Phosphatase or ACPP is part of a family of proteins called histidine acid phosphatase. ACPP enhances the hydrolyzation of many phosphate monoesters and proteins that are phosphorylated. In order to function best, ACPP needs a range of range of 4-6 pH, furthermore, L(+)-tartrate inhibits ACPP’s catalyzation. This enzyme can act as a lipid phosphatase as well and can inhibit lysophosphatidic acid in seminal plasma.
DESCRIPTION
ACPP Mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 356 amino acids (32-381 aa) and having a molecular mass of 41.3kDa.
ACPP is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
SOURCE
PHYSICAL APPEARANCE
FORMULATION
The ACPP solution (0.5mg/ml) contains 10% Glycerol and Phosphate-Buffered Saline (pH 7.4).
STABILITY
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
PURITY
Greater than 95.0% as determined by SDS-PAGE.
BIOLOGICAL ACTIVITY
Specific activity is >80,000 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of pnitrophenyl phosphate (pN/.P-P) per minute at pH 5.0 at 37C.
AMINO ACID SEQUENCE
KELKFVTLVF RHGDRGPIET FPTDPITESS WPQGFGQLTQ WGMEQHYELG SYIRKRYGRF LNDTYKHDQI YIRSTDVDRT LMSAMTNLAA LFPPEGISIW NPRLLWQPIP VHTVSLSEDR LLYLPFRDCP RFEELKSETL ESEEFLKRLH PYKSFLDTLS SLSGFDDQDL FGIWSKVYDP LFCESVHNFT LPSWATEDAM IKLKELSELS LLSLYGIHKQ KEKSRLQGGV LVNEILKNMK LATQPQKYKK LVMYSAHDTT VSGLQMALDV YNGVLPPYAS CHMMELYHDK GGHFVEMYYR NETQNEPYPL TLPGCTHSCP LEKFAELLDP VISQDWATEC MATSSHQGRN HHHHHH.
SAFETY DATA SHEET
SDS
USAGE
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文献和实验ACPP Mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 356 amino acids (32-381 aa) and having a molecular mass of 41.3kDa.
ACPP is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
9. Wash in buffer. 3 minutes. 10. Incubate in rabbit anti-mouse immunoglobins. 30 minutes. 11. Jet wash in buffer. 12. Wash in buffer. 3 minutes. 13. Incubate in alkaline phosphatase-mouse anti-alkaline phosphatase 30 minutes. 14. Jet
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
. Biol. In press, 1994. *Fields-Berry, S.C., A.L. Halliday, and C.L. Cepko. 1992. A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina. Proc. Natl. Acad. Sci. USA. 89
on the spermatic cord. They should not be cut prior to fixation because they will rupture, instead fix them whole. The prosate, in the mouse, contains several different lobes. The anterior prostate is a mat of soft tissue which lies tucked into proximal fold
技术资料
