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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 英文名:
β - galactosidase (β –GAL) Activity Assay Kit
- 库存:
现货
- 供应商:
武汉益普生物科技有限公司
- 规格:
96T(40 samples)
| 中文名称 |
β-半乳糖苷酶(β-GAL)比色法测试盒
|
| 英文名称 |
β - galactosidase (β –GAL) Activity Assay Kit
|
| 产品货号 |
E-BC-K631-M
|
| 检测原理 |
β-半乳糖苷酶能够催化底物PNPG(对硝基 苯基-β-D-吡喃半乳糖苷)生成对硝基 苯酚,对硝基 苯酚在400 nm有最大吸收峰,通过测定在单位时间内吸光度值的变化量来计算β-GAL的酶活。
|
| 灵敏度 |
16.19 U/L
|
| 检测范围 |
16.19-1200 U/L
|
| 精密度 |
批间差:6.0% | 批内差:3.0%
|
| 检测时间 |
1 h 10 min
|
| 检测仪器及波长 |
酶标仪(390-410 nm,最佳检测波长为400 nm)
|
| 研究领域 |
Amino acids proteins
|
| 样本类型 |
血清(浆);动植物组织;细胞
|
| 结果类型 |
酶活
|
| 方法 |
比色法
|
| 保存及保质期 |
该产品可在 2~8°C/-20°C避光条件下保存6个月,具体试剂组分的保存条件请参考说明书
|

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文献和实验β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer
method for assaying β-galactosidase activity in lysates prepared from cells transfected with β-galactosidase reporter vectors such as Promega's pSV-β-Galactosidase Control Vector. The standard assay is performed by adding a dilute sample
Digital Image Analysis of Cells Stained with the Senescence-Associated -Galactosidase Assay
-associated β-galactosidase (SABG) activity. Here we describe a procedure for digital image analysis of cells stained by the SABG staining technique at pH 6 or at pH 4. This analysis is highly reproducible and sensitive to subtle differences in staining
Multiplex Lysosomal Enzyme Activity Assay on Dried Blood Spots Using Tandem Mass Spectrometry
occur leads to better clinical outcomes. In the method which follows, lysosomal α-glucosidase, α-galactosidase, β-glucocerebrosidase, acid sphingomyelinase, and galactocerebrosidase are extracted from dried blood spots and incubated individually
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