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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2~8°C
- 保质期:
12个月
- 英文名:
α-Amylase Activity Assay Kit
- 库存:
现货
- 供应商:
武汉益普生物科技有限公司
- 规格:
96T(40 samples)
| 中文名称 |
α-淀粉酶比色法测试盒
|
| 英文名称 |
α-Amylase Activity Assay Kit
|
| 产品货号 |
E-BC-K007-M
|
| 检测原理 |
还原糖与 3,5-二硝基水杨酸在加热条件下反应,生成棕红色物质,利用β-淀粉酶不耐热的特性,将其钝化,然后测定α-淀粉酶的酶活。
|
| 灵敏度 |
0.0097 U/mL
|
| 检测范围 |
0.0097-0.3474 U/mL
|
| 精密度 |
批间差:3.9% | 批内差:3%
|
| 检测时间 |
40 min
|
| 检测仪器及波长 |
酶标仪(535-540 nm,最佳检测波长540 nm)
|
| 研究领域 |
other;Plant Physiology Series
|
| 样本类型 |
血清(浆);唾液;植物;动物组织
|
| 结果类型 |
酶活
|
| 方法 |
比色法
|
| 保存及保质期 |
该产品可在 2~8°C避光条件下保存12个月
|

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文献和实验High-Throughput Screening of Mutant -Amylase Libraries for Increased Activity at 129C
We describe a high throughput screening setup for measuring α-amylase activity at temperatures far above the boiling point of water. The system consists of a sealed aluminum 384-well assay plate incubated between two preheated aluminum blocks
Assay of Mammalian S-Adenosylmethionine Decarboxylase Activity
to exist as an α2 β2 heterotetramer (3 ). In addition, the small subunit has been shown to contain residues essential for catalytic activity (4 ).
Generation of Osteoclasts In Vitro, and Assay of Osteoclast Activity
are formed in response to various osteotropic factors such as 1α,25-dihydroxyvitamin D3 , parathyroid hormone, prostaglandin E2 , and interleukin -11. Recent studies have revealed that osteoblasts express two cytokines essential for osteoclastogenesis
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