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文献和实验. Immerse the portion of the tubes containing the cytosol in a 37oC water bath and allow MT protein to polymerize into MTs by incubating for 45 minutes. During this polymerization incubation, warm the ultracentrifuge and 50.2Ti rotor to 25oC
Porcine brain tubulin preparation
the cytosol in a 37℃ water bath and allow MT protein to polymerize into MTs by incubating for 45 minutes. 12. During this polymerization incubation, warm the ultracentrifuge and 50.2Ti rotor to 25℃. 13. Pellet MTs from the cytosol by centrifugation
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College Exercise 10.3 - Extraction of Chromatin LEVEL II Materials Bovine or porcine brain 0.25 M Sucrose containing 0.0033 M calcium
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