Click-&-Go® Dde 蛋白富集试剂盒-Click-

Click-&-Go® Dde 蛋白富集试剂盒，Click-&-Go® Dde Protein Enrichment Kit，Click-&-Go® Dde 蛋白富集试剂盒,Click-&-Go® Dde Protein Enrichment Kit

产品名称：Click-&-Go® 蛋白富集试剂盒-Click-&-Go® Dde Protein Enrichment Kit

产品货号：CCT-1152

产品规格：1Kit

Click-&-Go® Dde蛋白质富集试剂盒是一种高效的、不含生物素/链霉亲和素的工具，用于通过点击反应在可切割琼脂糖树脂上捕获偶氮修饰的生物分子，并在温和条件下释放。通过点击化学方法，可以从复杂的蛋白质提取物中捕获偶氮修饰的蛋白质或其翻译后修饰形式。一旦通过铜催化点击化学与树脂共价结合，颗粒可以以高强度洗涤，几乎消除任何非特异性结合的蛋白质。用2%的肼处理后，可以得到高度富集的新生蛋白质。捕获的蛋白质可以经蛋白酶消化，得到非常纯净的肽片段池，非常适合质谱分析（如LC MS/MS）。

Click-&-Go® DDe Protein Enrichment Kit is an efficient, biotin/streptavidin-free tool for capturing azide-modified biomolecules on a cleavable agarose resin via click reaction and its subsequent release under mild conditions. Azide-modified proteins, or their post-translationally modified forms, are captured from complex protein extracts on the cleavable alkyne agarose resin supplied. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with high stringency virtually eliminating any non-specifically bound proteins. Treatment with 2% hydrazine yields a highly enriched population of nascent proteins. Captured proteins can be protease digested to yield a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis.The biotin-free enrichment improves signal to noise by eliminating nonspecific binding and increasing selectivity, thus improving detection of low abundant proteins. This approach is fully compatible with widely used MS techniques including iTRAC and ICAT.The biotin-free, covalent capture approach is superior to biotin or lectin based enrichment approaches, and ideal for proteomics, biomarker discovery, posttranslational modification (PTM) analysis, and more.

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点击：Click-&-Go® 蛋白富集试剂盒-Click-&-Go® Dde Protein Enrichment Kit查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides（Biotin-Azides）试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。，请点击查看艾美捷特色产品中心。