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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C.
- 保质期:
2年
- 英文名:
MB 488 NHS ester-25 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
MB 488 NHS ester-25mg,MB 488 NHS ester-25 mg,MB 488 NHS ester-25mg,MB 488 NHS ester-25 mg
MB 488 NHS ester-25mg-MB 488 NHS ester-25 mg
产品货号:CCT-FP-1601-25
产品规格:25mg
MB 488 NHS酯是一种非常明亮的亲水性绿色荧光染料,用于通过伯胺(Lys)标记蛋白质和抗体。

MB™ 488 is a very hydrophilic, water-soluble green emitting dye. Structurally related to Alexa Fluor® 488 dye, MB™ 488 dye is a next step in improving water solubility, brightness and minimizing self-quenching. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and very high photostability. MB™ 488 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally MB™ 488 is an excellent dye for flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many other applications. The NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

MB 488 NHS ester-25mg-MB 488 NHS ester-25 mg-20°C.
点击:MB 488 NHS ester-25mg-MB 488 NHS ester-25 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验振荡器混匀,磁珠用量根据所需取用,置于EP管中,加入偶联液,使磁珠的终浓度为5mg/mL,使用旋涡振荡器混匀,后置于磁性分离架上,富集磁珠,去除上清,后再加入偶联液重复清洗一次。 2.3. 计算所需SA的体积,先加入偶联液,旋涡震荡混匀,再加入EDC和NHS,最后加入SA,然后继续旋涡混匀,后置于37℃环境旋转混匀反应2h。EDC与NHS的比例为1:1,此偶联反应中,EDC和NHS的终浓度都是1.5mg/ml,SA与磁珠的比例为1:25,磁珠的终浓度亦为5mg/mL。 2.4. 偶联反应完成
Labeling Tubulin and Quantifying Labeling Stoichiometry
that are not clear, using DMSO instead of glycerol for the first polymerization step appears to increase the labeling stoichiometry by ~25% for C2CF-SNHS ester. For labeling with rhodamine (tetramethylrhodamine NHS ester) and X-rhodamine, we generally use glycerol
Coupling Peptides To Resin For Affinity Purification
for 10 min at RT. The resin has about 10 5mol groups/ml, so add about 20 5mol reagent per ml (7 mg/ml resin). Dissolve the IAA-NHS ester in dry DMSO and add it while stirring the resin. This step and subsequent steps up to the blocking of residual
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