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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C.
- 保质期:
2年
- 英文名:
IR 680LT NHS ester-100 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
IR 680LT NHS酯-100 mg,IR 680LT NHS ester-100 mg,IR 680LT NHS酯-100 mg,IR 680LT NHS ester-100 mg
IR 680LT NHS酯-100 mg-IR 680LT NHS ester-100 mg
产品货号:CCT-FP-1526-100
产品规格:100mg
IR Fluor 680(相当于IRDye® 680)是一种明亮的近红外荧光胺反应性染料,用于标记蛋白质的伯胺。

IR 680LT (IRDye® 680LT equivalent) is a bright, water-soluble dye that is structurally very similar to the commonly used Cy®5.5 Dye. The water solubility, brightness, and self-quenching characteristics of this dye were significantly improved by the introduction of two additional sulfo groups. Far-red fluorescent IR 680 conjugates are spectrally similar to other 680 nm dyes that are used for flow cytometry, microscopy and other applications. Proteins can be labeled with IR 680 at high molar ratios without significant self - quenching, leading to brighter conjugates and more sensitive detection. The NHS ester (succinimidyl ester) is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

IR 680LT NHS酯-100 mg-IR 680LT NHS ester-100 mg-20°C.
点击:IR 680LT NHS酯-100 mg-IR 680LT NHS ester-100 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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与离心管一起,10000rpm,30s,DNA到EP管,弃柱子; 11. 加50ul 水到minicolumn 室温下静置5min 12. 离心20s 收积洗提液,弃掉minicolumn 13. 加5.5ul 3M NaOH 然后 37℃ 孵育15 min 14. 短暂离心 15. 加 1ul 糖原(10mg/ml) 16. 加33.3ul 5M NH4OAcetate PH 7.0 17. 加330ul 冷(-20℃)100%乙醇,充分混匀 18. -20℃过夜
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