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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C.
- 保质期:
2年
- 英文名:
Cy3 Maleimide-25 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
Cy 3 Maleimide 25mg,Cy3 Maleimide-25 mg,Cy 3 Maleimide 25mg,Cy3 Maleimide-25 mg
Cy 3 Maleimide 25mg-Cy3 Maleimide-25 mg
产品货号:CCT-FP-1302-25
产品规格:25mg
Cy 3染料的马来酰亚胺衍生物是用于将染料缀合至抗体、含半胱氨酸的肽和其它含巯基的分子上的巯基的最流行的工具。马来酰亚胺基团在pH 6.5至7.5下特异性且有效地与还原的硫醇(巯基,-SH)反应以形成稳定的硫醚键。

The maleimide derivative of Cy3 dye is the most popular tool for conjugating the dye to a thiol group on antibodies, cysteine-containing peptides and other thiol-containing molecules. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds.Cy3 Amine (also sold under Sulfo-Cyanine3 Amine) is a bright, water-soluble, and pH insensitive from pH 4 to pH 10 orange-fluorescent dye. Cy3 conjugates of antibodies, peptides, and proteins can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.This sulfonated dye is also known as sulfo-Cyanine3 Maleimide. Cy®Dye is a trademark of GE Healthcare.

Cy 3 Maleimide 25mg-Cy3 Maleimide-25 mg-20°C.
点击:Cy 3 Maleimide 25mg-Cy3 Maleimide-25 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验and stability. Here we elaborate detailed protocols using maleimide-based chemistry for the conjugation to reduce hinge disulfides in antibodies by several cytotoxic drugs. We present a method for the initial characterization of the reduction/alkylation reaction
Amplification and Labelling with Cy dyes
dGTP 5ml 100mM dTTP 5ml 1XTE Reaction tube containing the following for 1-50ml reaction: 15ml PCR product from Step 2 5ml 10X PCR buffer 0.5ml F-100X dNTPs 1ml 50mM Primer 2 1ml 25mM MgCl2 24ml water 3ml Cy dye (add last) Program
报告单上获得。如果需要配制成其他浓度,按上述公式换算。注意: 1 OD260= 33 ug/ml.9. 如何计算引物的 Tm 值?答:引物设计软件都可以给出 Tm ,与引物长度、碱基组成、引物使用缓冲的离子强度有关。长度为 25mer 以下的引物, Tm 计算公式为:Tm = 4℃ (G + C)+ 2℃ (A + T)对于更长的寡聚核苷酸, Tm 计算公式为:Tm = 81.5 + 16.6 x Log10[Na+] + 0.41
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