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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C.
- 保质期:
2年
- 英文名:
AZDye 488 TFP Ester-25 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
AZDye 488 TFP酯-25 mg,AZDye 488 TFP Ester-25 mg,AZDye 488 TFP酯-25 mg,AZDye 488 TFP Ester-25 mg
AZDye 488 TFP酯-25 mg-AZDye 488 TFP Ester-25 mg
产品货号:CCT-FP-1026-25
产品规格:25mg
AZDye™ 488 TFP酯是一种胺反应性发绿光染料,通常用于通过伯胺(Lys)、胺修饰的寡核苷酸和其他含胺生物分子标记蛋白质或抗体。标记在pH 7-9时最有效,并形成稳定的共价酰胺键。AZDye™ 488缀合物是具有非常高的荧光量子产率的高度光稳定的染料。AZDye™ 488在结构上与Alexa Fluor® 488相同。它的吸收/发射光谱与许多其他基于磺化罗丹明110核心的荧光染料的光谱完美匹配,包括DyLight® 488,Alexa Fluor® 488。

AZDye™ 488 TFP Ester is an amine reactive, green emitting dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.AZDye™ 488 is a water-soluble, highly photostable dye with very high fluorescence quantum yield. Its green fluorescence is pH independent from pH 4 to pH 10. The excitation of AZDye™ ideally suited for the 488 nm line. AZDye™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.2,3,5,6-tetrafluorophenyl (TFP) esters is another type of carboxylic acid derivative that react with primary amines forming covalent amide bond. The amine linkage bond is identical to one formed by the reaction between primary amines and NHS esters or sulfo-NHS esters. However, in most cases, TFP ester displays much better stability toward hydrolysis in aqueous media resulting in more efficiency and better reproducible labeling of biopolymers.AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488 Dye.Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

AZDye 488 TFP酯-25 mg-AZDye 488 TFP Ester-25 mg-20°C.
点击:AZDye 488 TFP酯-25 mg-AZDye 488 TFP Ester-25 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验1. 引物是如何合成的?目前引物合成基本采用固相亚磷酰胺三酯法。 DNA 合成仪有很多种 , 主要都是由 ABI/PE 公司生产,而 Bioneer自行研制 的专利 384并行高通量 DNA合成仪, 可实现 99% 的高合成率。无论采用什么机器合成,合成的原理都相同,主要差别在于合成产率的高低,试剂消耗量的不同和单个循环用时的多少。亚磷酰胺三酯法合成 DNA 片段,具有高效、快速的偶联以及起始反应物比较稳定的特点。亚磷酰胺三酯法是将 DNA 固定在固相载体上完成
was recently described for a nanowire deposited on a metal film as an efficient platform for SERS (23 ). In this chapter we will compare the fluorescence enhancements on SIFs and SACS. We will also emphasize the advantages of SACS in a model Alexa488
one set of cells with 500 μg ml−1 dextran Alexa 488, 10 kDa (prepared at 1 mg ml−1 in internalization medium). Incubate a second set of cells with 500 μg ml−1 dextran Alexa 594, 10 kDa (prepared at 1 mg ml−1 in internalization medium
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