AZDye 488马来酰亚胺-25 mg-AZDye 488

AZDye 488马来酰亚胺-25 mg，AZDye 488 Maleimide-25 mg，AZDye 488马来酰亚胺-25 mg,AZDye 488 Maleimide-25 mg

AZDye 488马来酰亚胺-25 mg-AZDye 488 Maleimide-25 mg

产品货号：CCT-FP-1014-25

产品规格：25mg

AZDye™ 488马来酰亚胺是一种绿色荧光、水溶性染料，在pH 4至pH 10范围内具有pH不敏感发射。这种染料非常适合488 nm激光线。AZDye 488是市售最亮的染料之一，荧光量子产率接近100%。马来酰亚胺是用于将染料缀合至蛋白质上的硫醇基团、寡核苷酸硫代磷酸酯或低分子量配体的最流行的巯基反应性反应基团。马来酰亚胺基团在pH 6.5至7.5下特异性且有效地与还原的硫醇(巯基，-SH)反应以形成稳定的硫醚键。所得的共轭物比许多其他光谱相似的荧光团的共轭物表现出更亮的荧光和更大的光稳定性。

AZDye™ 488 Maleimide (Alexa Fluor® 488 C5 Maleimide equivalent) is a thio reactive, green emitting dye routinely used to label proteins or antibodies through the sulfhydryl groups (Cys), thoil-modified oligonucleotides, and other thiol-containing biomolecules. The labeling occurs most efficiently at pH 6.5-7.5 and forms a stable, covalent amide bond.AZDye™ 488 is a water-soluble, highly photostable dye with very high fluorescence quantum yield. Its green fluorescence is pH independent from pH 4 to pH 10. The excitation of AZDye™ ideally suited for the 488 nm line. AZDye™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488. AZDye™ 488 Maleimide can be used a less expensive alternative to Alexa Fluor® 488 C5 Maleimide.Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.

AZDye 488马来酰亚胺-25 mg-AZDye 488 Maleimide-25 mg-20°C.

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