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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C.
- 保质期:
2年
- 英文名:
AZDye 488 NHS Ester-25 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
AZDye 488 NHS酯-25 mg,AZDye 488 NHS Ester-25 mg,AZDye 488 NHS酯-25 mg,AZDye 488 NHS Ester-25 mg
AZDye 488 NHS酯-25 mg-AZDye 488 NHS Ester-25 mg
产品货号:CCT-FP-1013-25
产品规格:25mg
AZDye™ 488 NHS酯是一种胺反应性、发射绿色的染料,通常用于通过伯胺(Lys)、胺修饰的寡核苷酸和其他含胺生物分子标记蛋白质或抗体。标记在pH 7-9时最有效,并形成稳定的共价酰胺键。AZDye™ 488在结构上与Alexa Fluor® 488相同。它的吸收/发射光谱与许多其他基于磺化罗丹明110核心的荧光染料的光谱完美匹配,包括DyLight® 488,Alexa Fluor® 488。AZDye™ 488 NHS酯可用作Alexa Fluor® 488 NHS酯的替代品。

AZDye™ 488 NHS Ester is an amine reactive, green emitting dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.AZDye™ 488 is a water-soluble, highly photostable dye with very high fluorescence quantum yield. Its green fluorescence is pH independent from pH 4 to pH 10. The excitation of AZDye™ ideally suited for the 488 nm line. AZDye™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488. AZDye™ 488 NHS Ester can be used a less expensive alternative to Alexa Fluor® 488 NHS Ester.Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.

AZDye 488 NHS酯-25 mg-AZDye 488 NHS Ester-25 mg-20°C.
点击:AZDye 488 NHS酯-25 mg-AZDye 488 NHS Ester-25 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Zymolyase Dissolve in 10% glucose. Store aliquots (25 µl) at 20o C in common freezer for stock solutions Use once and dispose Comments: alternative is to dissolve in SCE, 40% gycerol, 20 mg/ml final. Can use
人 β2-微球蛋白(β2-MG) 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中 β2-微球蛋白(β2-MG)的 含量。 实验原理 : 本试剂盒应用 双抗体 夹心法测定 标本 中 人β2- 微球蛋白(β 2-MG ) 水平。用纯化的 人β2- 微球蛋白(β 2-MG )抗体 包被微孔板,制成固相 抗 体,往包被 单抗 的微孔中依次加入 β2- 微球蛋白(β
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
50% glycerol100 mM Tris-HCl pH8 @ 25 degrees 100 microliters 1 M Tris pH8300 mM KCl 300 microliters 1 M KCl25 mM MgCl2 25 microliters 1 M MgCl2500 micrograms/ml BSA 5 microliters 100 mg/ml BSA170 microliters H2Ooptional: add 25 microliters saturated
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