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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C.
- 保质期:
2年
- 英文名:
Cy3 Alkyne-25 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
Cy 3 Alkyne-25mg,Cy3 Alkyne-25 mg,Cy 3 Alkyne-25mg,Cy3 Alkyne-25 mg
Cy 3 Alkyne-25mg-Cy3 Alkyne-25 mg
产品货号:CCT-TA117-25
产品规格:25mg
Cy 3炔可以通过铜催化的点击反应(CuAAC)与叠氮化物反应,形成稳定的三唑,并且不需要升高的温度。这种红色荧光探针是水溶性的,并且其荧光在pH 4至pH 10之间是pH不敏感的。其激发峰非常适合532 nm或555 nm激光线,其吸收和发射光谱与Alexa Fluor® 555、CF® 555染料或任何其他基于Cyanine 3的荧光染料几乎相同。

Cy3 Alkyne can be reacted with azides via a copper-catalyzed click reaction (CuAAC) forming a stable triazole and does not require elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets. Cy3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine5 based fluorescent dyes. This is sulfonated dye is also known as sulfo-Cyanine3. Cy®Dye is a trademark of GE Healthcare. Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

Cy 3 Alkyne-25mg-Cy3 Alkyne-25 mg-20°C.
点击:Cy 3 Alkyne-25mg-Cy3 Alkyne-25 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Amplification and Labelling with Cy dyes
dGTP 5ml 100mM dTTP 5ml 1XTE Reaction tube containing the following for 1-50ml reaction: 15ml PCR product from Step 2 5ml 10X PCR buffer 0.5ml F-100X dNTPs 1ml 50mM Primer 2 1ml 25mM MgCl2 24ml water 3ml Cy dye (add last) Program
报告单上获得。如果需要配制成其他浓度,按上述公式换算。注意: 1 OD260= 33 ug/ml.9. 如何计算引物的 Tm 值?答:引物设计软件都可以给出 Tm ,与引物长度、碱基组成、引物使用缓冲的离子强度有关。长度为 25mer 以下的引物, Tm 计算公式为:Tm = 4℃ (G + C)+ 2℃ (A + T)对于更长的寡聚核苷酸, Tm 计算公式为:Tm = 81.5 + 16.6 x Log10[Na+] + 0.41
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Direct labeling of total RNA with Cy3 and Cy5: A. MATERIALS RNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat # 18064-014) QIAquick PCR Purification Kit (Qiagen; Cat # 28106)
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