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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C. Desiccate
- 保质期:
2年
- 英文名:
Biotin-PEG3-Azide-5 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
生物素-PEG 3-Azide-5 mg,Biotin-PEG3-Azide-5 mg,生物素-PEG 3-Azide-5 mg,Biotin-PEG3-Azide-5 mg
生物素-PEG 3-Azide-5 mg-Biotin-PEG3-Azide-5 mg
产品货号:CCT-AZ104-5
产品规格:5mg
生物素-PEG 3-叠氮化物是不可裂解的叠氮化物活化生物素化试剂,其通过铜催化的点击反应与末端炔反应,通过无铜点击反应与应变环辛炔(例如DBCO或BCN化合物)反应,或通过称为Staudinger化学的机制与膦标记的分子反应,从而实现生物样品中衍生分子的有效和特异性缀合。这些反应是生物正交的,或者换句话说,与生物系统相容,因为它的组分不与生物环境反应。

Biotin-PEG3-Azide is non-cleavable, azide-activated biotinylation reagent that reacts with terminal alkynes via a copper-catalyzed click reaction to produce a stable triazole linkage. It also reacts with cyclooctynes via a copper-free "click chemistry" reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This reaction is bioorthogonal or in other words compatible with biological systems in that it's components do not react with the biological environment. This reagent is also know as Biotin-Azide.For the detection of low abundance targets or where significant increase in signal intensity is desired please consider using next generation azides probes containing an internal copper-chelating motif.

生物素-PEG 3-Azide-5 mg-Biotin-PEG3-Azide-5 mg-20°C. Desiccate
点击:生物素-PEG 3-Azide-5 mg-Biotin-PEG3-Azide-5 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Oxalyticase Dissolve in 0.1 M NaCl, 0.02% Na azide, 50% glcyerole Store aliquots (1 ml) at 20o C in common freezer for stock solutions 上一篇:ALPHA FACTOR ARREST AND RELEASE 下一篇:GLASS BEADS
PEG Preparation of Plasmid DNA
PEG Preparation of Plasmid Plasmid isolated by this procedure can be used routinely
10g/L牛血清白蛋白(W/W)PBS液 分子筛柱(如G25) 电磁搅拌器 透析袋(MW CO 8000) 铝铂 微量移液器 1. 将待生物素化的蛋白质用0.1 mol/L 碳酸氢钠缓冲液(pH 8.0)或0.5 mol/L 硼酸缓冲液(pH 8.6)稀释到1 mg/ml,一般实验室应用的生物素化体积为1~2.5 ml。 2. 交互用0.1 mol/L 碳酸氢钠缓冲液(pH 8.0)或0.5 mol/L 硼酸缓冲液(pH 8.6),对蛋白质充分透析。
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