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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C.
- 保质期:
2年
- 英文名:
6,8-Difluoro-7-hydroxy-4-methylcoumarin NHS Ester-100 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
6,8-二氟-7-羟基-4-甲基香豆素NHS酯-100 mg,6,8-Difluoro-7-hydroxy-4-methylcoumarin NHS Ester-100 mg,6,8-二氟-7-羟基-4-甲基香豆素NHS酯-100 mg,6,8-Difluoro-7-hydroxy-4-methylcoumarin NHS Ester-100 mg
6,8-二氟-7-羟基-4-甲基香豆素NHS酯-100 mg-6,8-Difluoro-7-hydroxy-4-methylcoumarin NHS Ester-100 mg
产品货号:CCT-FP-1242-100
产品规格:100mg
6,8-二氟-7-羟基-4-甲基香豆素NHS酯(Marina Blue® NHS Ester)是蓝色荧光7-羟基香豆素的高性能替代品。

6,8-Difluoro-7-hydroxy-4-methylcoumarin NHS Ester (Marina Blue® NHS Ester) exhibit bright blue fluorescence emission near 460 nm is optimally excited by the intense 365 nm spectra line of the mercury-arc lamp and detect optimally with DAPI optical filter sets. Because the pKa value of 6,8-Difluoro-7-hydroxy-4-methylcoumarin derivatives are 2-3 log units lower compared to those of the corresponding 7-hydroxycoumarin conjugates, 6,8-Difluoro-7-hydroxy-4-methylcoumarin conjugates are strongly fluorescent even at neutral pH. 6,8-Difluoro-7-hydroxy-4-methylcoumarin NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

6,8-二氟-7-羟基-4-甲基香豆素NHS酯-100 mg-6,8-Difluoro-7-hydroxy-4-methylcoumarin NHS Ester-100 mg-20°C.
点击:6,8-二氟-7-羟基-4-甲基香豆素NHS酯-100 mg-6,8-Difluoro-7-hydroxy-4-methylcoumarin NHS Ester-100 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Coupling Peptides To Resin For Affinity Purification
for 10 min at RT. The resin has about 10 5mol groups/ml, so add about 20 5mol reagent per ml (7 mg/ml resin). Dissolve the IAA-NHS ester in dry DMSO and add it while stirring the resin. This step and subsequent steps up to the blocking of residual
Coupling Peptides To Resin For Affinity Purification
about 20 5mol reagent per ml (7 mg/ml resin). Dissolve the IAA-NHS ester in dry DMSO and add it while stirring the resin. This step and subsequent steps up to the blocking of residual iodoacetate groups should be done in dim light since the iodo group
Revised amino-allyl labeling protocol
) 8 m L 100 mM aminoallyl-dUTP** (Sigma, #A0410) 2 m L 100 mM dTTP **Dissolve 10 mg aminoallyl-dUTP in 170 m L water. Add approx. 6.8 m L 1N NaOH. Final pH is roughly
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