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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C.
- 保质期:
2年
- 英文名:
Difluorocarboxyfluorescein NHS Ester, 6-isomer-5 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
二氟羧基荧光素NHS酯,6-异构体-5 mg,Difluorocarboxyfluorescein NHS Ester, 6-isomer-5 mg,二氟羧基荧光素NHS酯,6-异构体-5 mg,Difluorocarboxyfluorescein NHS Ester, 6-isomer-5 mg
二氟羧基荧光素NHS酯,6-异构体-5 mg-Difluorocarboxyfluorescein NHS Ester, 6-isomer-5 mg
产品货号:CCT-FP-1223-5
产品规格:5mg
二氟羧基荧光素NHS酯,6-异构体(俄勒冈州绿色488 NHS酯当量)是一种明亮的绿色荧光发射染料。

Difluorocarboxyfluorescein NHS Ester, 6-isomer (also known as Oregon Green™ 488 Carboxylic Acid, Succinimidyl Ester, 6-isomer) is an amine-reactive fluorinated analog of fluorescein that overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa (pKa ~ 4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range. Although the mixed isomers of Difluorocarboxyfluorescein NHS Ester, is a preferred, routinely used fluorescent dye for labeling proteins, peptides and nucleotides, purification of peptide and nucleotides labeled with 5(6) isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer usually give better resolution in HPLC purification that is often required in the conjugation processes. Difluorocarboxyfluorescein NHS Ester dye is structurally identical toOregon Green™ 488 Carboxylic Acid, Succinimidyl Ester, 6-isomer sold by ThermoFisher Scientific.

二氟羧基荧光素NHS酯,6-异构体-5 mg-Difluorocarboxyfluorescein NHS Ester, 6-isomer-5 mg-20°C.
点击:二氟羧基荧光素NHS酯,6-异构体-5 mg-Difluorocarboxyfluorescein NHS Ester, 6-isomer-5 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验RNA Purification Protocol for 100-200 mg of Animal Tissue
设备 1. Centrifuge capable of 3,000-5,000 x g 2. High speed centrifuge tube with capacity of at least 15 ml 3. Ice bath 4. Ground pestle 实验步骤 1. Dissect tissue quickly and freeze in liquid nitrogen
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
ratio.Dilute 1:40 with the dialysis buffer and read the OD at 495 nm. D/P = {OD495 x 41 / 60,000} / {(mg/ml)/ 43,000} 8.Dilute to 3-5 mg/ml with the dialysis buffer.Calculate total mg of actin.Drop freeze in liquid N2 after dissolving 2 mg sucrose
Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
fresh 10 mg/ml stock in DMSO.Need 2.5 mg per 50 mg of tubulin. 8.Potassium glutamate,1 M.Prepare 1 M glutamic acid and titrate with KOH to pH 6.9. 9.Injection Buffer: 50 mM potassium glutamate,0.5 mM MgCl2,pH 6.5-6.7.Prepare 10 ml.Keep ~5 ml at 37℃
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