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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C.
- 保质期:
2年
- 英文名:
AZDye 594 TFP Ester-100 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
AZDye 594 TFP酯-100 mg,AZDye 594 TFP Ester-100 mg,AZDye 594 TFP酯-100 mg,AZDye 594 TFP Ester-100 mg
AZDye 594 TFP酯-100 mg-AZDye 594 TFP Ester-100 mg
产品货号:CCT-FP-1111-100
产品规格:100mg
AZDye™ 594 TFP酯是一种胺反应性红色荧光染料,通常用于通过伯胺(Lys)、胺修饰的寡核苷酸和其他含胺生物分子标记蛋白质或抗体。AZDye™ 594染料可用于高摩尔比的蛋白质标记,而不会发生显著的自猝灭,从而实现更明亮的缀合物和更灵敏的检测。

AZDye™ 594 TFP Ester is an amine-reactive, red fluorescent dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. AZDye™ 594 dye can be used for proteins labeling at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.AZDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AZDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.A2,3,5,6-tetrafluorophenyl (TFP) esters is another type of carboxylic acid derivative that react with primary amines forming covalent amide bond. The amine linkage bond is identical to one formed by the reaction between primary amines and NHS esters or sulfo-NHS esters. However, in most cases, TFP ester displays much better stability toward hydrolysis in aqueous media resulting in more efficiency and better reproducible labeling of biopolymers.AZDye™ 594 is structurally identical to Alexa Fluor® 594.Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

AZDye 594 TFP酯-100 mg-AZDye 594 TFP Ester-100 mg-20°C.
点击:AZDye 594 TFP酯-100 mg-AZDye 594 TFP Ester-100 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验one set of cells with 500 μg ml−1 dextran Alexa 488, 10 kDa (prepared at 1 mg ml−1 in internalization medium). Incubate a second set of cells with 500 μg ml−1 dextran Alexa 594, 10 kDa (prepared at 1 mg ml−1 in internalization medium
免疫共沉淀(Co-Immunoprecipitation)是以抗体和抗原之间的专一性作用为基础的用于研究蛋白质相互作用的经典方法。是确定两种蛋白质在完整细胞内生理性相互作用的有效方法。 注意:在准备做磷酸(酯)酶实验的时候,不加磷酸酯酶抑制剂。 一、工作液配制 配制 100 ml 的 modified RIPA buffe: 1.称取 790 mg 的 Tris-Base,加到 75 ml 去离子水中,加入 900 mg 的 NaCl,搅拌
)配制浓度至少为1 ~3mg/ml 如果在抗体储存过程中添加叠氮钠,则必须在硼酸盐缓冲液中充分透析去除叠氮钠。 3.按25~100μg/mg将生物素酯加入抗体,在室温下均匀混合孵化4h。在完成组合反应之前DMSO最终浓度不得低于5 %,否则生物素酯会沉淀。高浓度的生物素酯会导致体上结合多个生物素分子,因此所有抗体都可能被标记。较低的比例将使生物素化保持在最低限度(25μg生物素酯/mg摩尔比最初是抗体10:1)。 4.每250μg添加生物素酯20μl1mol/L氯化铵,室温孵化10min
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