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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C. Desiccate
- 保质期:
2年
- 英文名:
AZDye 546 Tyramide-150 slides
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
AZDye 546酪胺-150片,AZDye 546 Tyramide-150 slides,AZDye 546酪胺-150片,AZDye 546 Tyramide-150 slides
AZDye 546酪胺-150片-AZDye 546 Tyramide-150 slides
产品货号:CCT-1539-1
产品规格:150slides
橙子荧光试剂,广泛用于IHC、ICC、FISH和FISH中的酪胺信号放大(TSA)。酪胺信号放大(TSA)已被证明是一种特别通用和强大的酶放大技术,具有更高的检测灵敏度。TSA是基于HRP在低浓度过氧化氢存在下将标记的含酪胺底物转化为氧化的高反应性自由基的能力,该自由基可与HRP处或附近的酪氨酸残基共价结合。AZDye™ 546酪胺(Alexa Fluor® 546酪胺等效物)是许多酪胺信号放大试剂盒的组分,包括SuperBoost®。该试剂可与任何与HRP偶联的抗体一起用于酪胺信号放大,以在多重荧光免疫细胞化学(ICC)、免疫组织化学(IHC)和原位杂交(ISH)中检测低丰度靶标。

MB™ 543 DBCO is an orange colored fluorescent probe with absorption and emission maxima at 544 and 560 nm, respectively. The dye is based on a novel class of rhodamine dyes. Unlike traditional rhodamine dyes, MB™ 543 is not only bright and photostable but also highly water-soluble and pH-insensitive. MB™ 543 fluorescent dyes can be used in place of TAMRA, Alexa Fluor 546®, DyLight 546®, CF™543 and spectrally similar dyes.MB™ 543 DBCO dye reacts with azides via a copper-free "click chemistry" reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern MB™ 543 DBCO is an ideal alternative to copper requiring fluorescent alkynes.MB™ 543 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.DBCO reagents are the most commonly used substrates for strain-promoted copper-free azide-alkyne click chemistry reactions. DBCO compounds react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage.

AZDye 546酪胺-150片-AZDye 546 Tyramide-150 slides-20°C. Desiccate
点击:AZDye 546酪胺-150片-AZDye 546 Tyramide-150 slides查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
Introduction Basic Protocol 1: Production of ELISA Microarray Slides and Their Use in Assay for 3‐Nitrotyrosine Basic Protocol 2: Generation of 3‐Nitrotyrosine Standards by Modification of BSA
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