塔姆拉生物素炔-5mg-TAMRA Biotin Alkyn

塔姆拉生物素炔-5mg，TAMRA Biotin Alkyne-5 mg，塔姆拉生物素炔-5mg,TAMRA Biotin Alkyne-5 mg

塔姆拉生物素炔-5mg-TAMRA Biotin Alkyne-5 mg

产品货号：CCT-1366-5

产品规格：5mg

虽然生物素是一种高效的亲和标记，但荧光标记提供了一种更灵敏、定量和方便的蛋白质可视化方法。三功能点击化学探针结合了连接手柄、生物素和直接延伸的荧光团，以联合收割机结合两种类型标记的互补益处。双标记TAMRA生物素炔探针可以通过CuAAC连接容易地掺入叠氮化物标记的生物分子中。例如，双标记的新合成的蛋白质、聚糖、脂质或DNA/RNA可以通过亲和纯化容易地分离并通过凝胶内荧光可视化。

Whilst biotin is a highly effective affinity label, fluorescent labels provide a more sensitive, quantitative, and convenient method for visualizing proteins. Trifunctional click chemistry probes that incorporate a ligation handle, a biotin and a fluorophore straightforwardly extended to combine the complementary benefits of both types of label. Dual-label TAMRA Biotin Azide probe can be readily incorporated into alkyne-tagged biomolecules through CuAAC ligation. For example, dual-labeled newly synthesized proteins, glycans, lipids, or DNA/RNA can be readily isolated by affinity purification and visualized by in-gel fluorescence. Another very important advantage of dual label probes over regular biotin probes is built-in control. Each step of enrichment process can easily followed either by UV-Vis (550 nm) or by more sensitive fluorescence spectroscopy. After elution form streptavidin beads target proteins containing TAMRA label can be easily distinguished from non-specifically bound proteins and endogenously biotinlytated proteins. This new dual label probe should be useful to expand the new applications of standard biotin–streptavidin technology.

塔姆拉生物素炔-5mg-TAMRA Biotin Alkyne-5 mg-20°C.

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