DADPS生物素炔-25mg-DADPS Biotin Al

DADPS生物素炔-25mg，DADPS Biotin Alkyne-25 mg，DADPS生物素炔-25mg,DADPS Biotin Alkyne-25 mg

DADPS生物素炔-25mg-DADPS Biotin Alkyne-25 mg

产品货号：CCT-1331-25

产品规格：25mg

该试剂含有通过含有可裂解DADPS接头的间隔臂与叠氮化物部分连接的生物素部分。捕获的生物分子可以有效地释放在温和的条件下(10%甲酸，0.5小时)和小分子片段(84.12 Da)留在标记的蛋白裂解后。

Extraordinary strength of the streptavidin-biotin interaction allows for efficient capturing of even highly dilute targets; however, it makes recovery of proteins from affinity resins challenging. Conventional methods to elute biotinylated proteins from immobilized avidin include the following: (i) denaturation of streptavidin by boiling the resin in a denaturing buffer that may include high concentrations of chaotropic salts, (ii) trypsin digestion of proteins while they are bound to the resin, or (iii) elution of proteins with excess free biotin. These protocols can co-elute contaminant proteins by releasing nonspecifically bound proteins and/or naturally biotinylated proteins concurrently with labeled proteins. In addition, some of these methods can cause elution of high levels of resin-based peptides along with the proteins of interest, resulting in further sample contamination.DADPS (dialkoxydiphenylsilane) Biotin Alkyne probes eliminate a major limitation of the streptavidin-biotin affinity purification. This reagent contains a biotin moiety linked to an azide moiety through a spacer arm containing a cleavable DADPS linker. Captured biomolecules can be efficiently released under mild conditions (10% formic acid, 0.5 h) and the small (84 Da) molecular fragment left on the labeled protein following cleavage. These features make the DADPS probe especially attractive for use in biomolecular labeling and proteomic studies.

DADPS生物素炔-25mg-DADPS Biotin Alkyne-25 mg-20°C.

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