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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C. Desiccate
- 保质期:
2年
- 英文名:
AZDye 647 Azide-1 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
AZDye 647 Azide-1 mg,AZDye 647 Azide-1 mg,AZDye 647 Azide-1 mg,AZDye 647 Azide-1 mg
AZDye 647 Azide-1 mg-AZDye 647 Azide-1 mg
产品货号:CCT-1299-1
产品规格:1mg
AZDye™ 647 Azide(以前以AFDye™ 647 Azide标签销售)是一种水溶性、pH值不敏感(pH 4至pH 10)的远红荧光探针。它的激发非常适合633 nm或647 nm的激光线。AZDye™ 647在结构上类似于Alexa Fluor® 647,并且在光谱上几乎与Cy 5染料、Alexa Fluor® 647、CF® 647染料或任何其他基于Cyanine 5的荧光染料相同。

AZDye™ 647 Azide is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AZDye™ 647 over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.AZDye™ 647 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes. AZDye™ 647 Azide can be used a less expensive alternative to Alexa Fluor® 647 Azide.Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

AZDye 647 Azide-1 mg-AZDye 647 Azide-1 mg-20°C. Desiccate
点击:AZDye 647 Azide-1 mg-AZDye 647 Azide-1 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Oxalyticase Dissolve in 0.1 M NaCl, 0.02% Na azide, 50% glcyerole Store aliquots (1 ml) at 20o C in common freezer for stock solutions 上一篇:ALPHA FACTOR ARREST AND RELEASE 下一篇:GLASS BEADS
Replication timing by density transfer
. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289
Replication timing by comparative hybridization
the culture to the 37o bath. 5. Once the culture has reached 37o C (it takes 10-15 min), add Pronase (0.01-0.05 mg/ml final conc) to release the cells from the alpha factor block. [You can add dry Pronase. I usually dissolve the Pronase in 5 ml
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