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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C. Desiccate
- 保质期:
2年
- 英文名:
AZDye 594 Azide-1 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
AZDye 594 Azide-1 mg,AZDye 594 Azide-1 mg,AZDye 594 Azide-1 mg,AZDye 594 Azide-1 mg
AZDye 594 Azide-1 mg-AZDye 594 Azide-1 mg
产品货号:CCT-1295-1
产品规格:1mg
AZDye™ 594叠氮化物是一种明亮的红色荧光叠氮化物激活探针,通过铜催化点击反应(CuAAC)与末端炔反应。它还通过无铜点击化学反应与应变环辛炔反应以形成稳定的三唑,并且不需要Cu催化剂或升高的温度。AZDye™ 594非常适合Ar-Kr混合气体激光器的561 nm和594 nm激光线。AZDye™ 594叠氮化物染料在结构上与Alexa Fluor® 594叠氮化物相同。它的吸收/发射光谱与许多其他基于磺化罗丹明核的荧光染料的光谱完美匹配,包括CF®594 Dye,DyLight® 594和Alexa Fluor® 594。

AZDye™ 594 Azide is a bright, red-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. AZDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AZDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry. AZDye™ 594 dye structurally is identical to Alexa Fluor® 594.Alexa Fluor® 594 is a registered trademark of Thermo Fisher Scientific.

AZDye 594 Azide-1 mg-AZDye 594 Azide-1 mg-20°C. Desiccate
点击:AZDye 594 Azide-1 mg-AZDye 594 Azide-1 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Oxalyticase Dissolve in 0.1 M NaCl, 0.02% Na azide, 50% glcyerole Store aliquots (1 ml) at 20o C in common freezer for stock solutions 上一篇:ALPHA FACTOR ARREST AND RELEASE 下一篇:GLASS BEADS
Replication timing by density transfer
. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289
Replication timing by comparative hybridization
the culture to the 37o bath. 5. Once the culture has reached 37o C (it takes 10-15 min), add Pronase (0.01-0.05 mg/ml final conc) to release the cells from the alpha factor block. [You can add dry Pronase. I usually dissolve the Pronase in 5 ml
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