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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C. Desiccate
- 保质期:
2年
- 英文名:
MB 660R Azide-1 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
MB 660 RAzide-1 mg,MB 660R Azide-1 mg,MB 660 RAzide-1 mg,MB 660R Azide-1 mg
MB 660 RAzide-1 mg-MB 660R Azide-1 mg
产品货号:CCT-1463-1
产品规格:1mg
MB 660 R是一种明亮且光稳定的远红染料,在远红和近红外之间的边界光谱区域中,在约685 nm处发射荧光。虽然最大吸收在约665 nm处,但该染料可被633或635 nm激光充分激发。MB 660 R染料是水溶性的,在pH 4至pH 10范围内对pH不敏感。MB 660 R是一种基于罗丹明的染料,与一般的罗丹明染料一样,它具有出色的光稳定性。MB 660 R的上级光稳定性和出色的亮度使其成为共焦显微镜和其他要求苛刻的应用的理想选择。

MB 660R Azide is a bright and very photostable probe routinely used for imaging of alkyne-containing biomolecules. MB 660R Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.MB 660R is a bright and photostable far-red dye that emits fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR. Although the absorption maximum is at around 665 nm, this dye can be sufficiently excited by the 633 or 635 nm laser. MB 660R dye is water soluble and pH-insensitive from pH 4 to pH 10. MB 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is exceptionally photostable . The superior photostability and excellent brightness of MB 660R make the dye an ideal choice for confocal microscopy and other demanding applications.MB 660R dye spectrally is almost identical to Alexa Fluor® 660 and CF® 660R Dye and can be used a less expensive alternative to these dyes.Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium.

MB 660 RAzide-1 mg-MB 660R Azide-1 mg-20°C. Desiccate
点击:MB 660 RAzide-1 mg-MB 660R Azide-1 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Replication timing by comparative hybridization
cells per time point. (Raffinose has not worked well for me, so I avoid it.) 2. Arrest the culture with alpha factor (200 nM final concentration for bar1 strains) when the OD660 is about 0.2-0.25. 3. When the cells have arrested (more than 95
Replication timing by density transfer
. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289
Intracellular Cytokine Staining Protocol
anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking.eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified
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