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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C. Desiccate
- 保质期:
2年
- 英文名:
AZDye 546 Picolyl Azide-5 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
AZDye 546吡啶甲基Azide-5 mg,AZDye 546 Picolyl Azide-5 mg,AZDye 546吡啶甲基Azide-5 mg,AZDye 546 Picolyl Azide-5 mg
AZDye 546吡啶甲基Azide-5 mg-AZDye 546 Picolyl Azide-5 mg
产品货号:CCT-1284-5
产品规格:5mg
AZDye ™ 546是水溶性的,并且在pH 4至pH 10范围内对pH不敏感,是橙色荧光染料,吸收和发射最大值分别在554和570 nm处。它可以与488 nm和532 nm激光线一起使用。AZDye ™ 546染料与各种抗体、肽、蛋白质、示踪剂和扩增底物缀合,通常用于在成像和流式细胞术中产生稳定信号。

AZDye™ 546 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported .In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.AZDye™ 546 is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AZDye™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AZDye 546吡啶甲基Azide-5 mg-AZDye 546 Picolyl Azide-5 mg-20°C. Desiccate
点击:AZDye 546吡啶甲基Azide-5 mg-AZDye 546 Picolyl Azide-5 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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研究过,反离子以扩散双电层形式存在。也有人制备多孔甲基丙烯酸盐离子交换剂,但多数人愿意使用商品化的交换剂。Sevec和Frechet[5]制得聚甲基丙烯酸缩水甘油酯共聚二甲基乙烯丙烯酸酯色谱柱(300×8mm)用于蛋白质离子交换的制备色谱。他们能做到一次进样到这种色谱柱分离300mg的蛋白质混合物。Gawdzrk和Matynia制备了一种交联的(p,p’―二羟基苯)―丙烷二环氧甘油醚和二乙烯苯的甲基丙烯酸酯的多孔共聚物作为一种最新的HPLC填料并且评价了其作为正相和反相分离的效果
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