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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C. Desiccate
- 保质期:
2年
- 英文名:
AZDye 350 Picolyl Azide-25 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
AZDye 350Azide吡啶-25 mg,AZDye 350 Picolyl Azide-25 mg,AZDye 350Azide吡啶-25 mg,AZDye 350 Picolyl Azide-25 mg
AZDye 350Azide吡啶-25 mg-AZDye 350 Picolyl Azide-25 mg
产品货号:CCT-1268-25
产品规格:25mg
AZDye™ 350 Picolyl Azide是一种先进的荧光探针,它结合了铜螯合基序,以提高反应位点处Cu(I)的有效浓度,从而提高CuAAC反应的效率,从而实现更快、更生物相容的CuAAC标记。据报道,与常规叠氮化物相比,信号强度增加高达40倍。

AZDye™ 350 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported .In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.AZDye™ 350 is a water-soluble moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line. It is routinely used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.

AZDye 350Azide吡啶-25 mg-AZDye 350 Picolyl Azide-25 mg-20°C. Desiccate
点击:AZDye 350Azide吡啶-25 mg-AZDye 350 Picolyl Azide-25 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Replication timing by density transfer
. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289
比妥钠,1ml~1.5ml/kg,但要体重称准。 5、戊巴比妥钠,40mg/kg,但不安全,用水合氯醛腹腔注射最安全,300mg/kg。或是用乌拉坦。用复合麻醉,2小时左右很有效。 配方: 戊巴比妥钠:0.886g 水合氯醛:4.25g 硫酸镁:2.12g 无水酒精:14.25ml 丙二醇:33.8ml 去离子水定容至100ml 用量:0.25ml/100g 6、2%戊巴比妥钠50mg/kg,书上推荐的剂量是40~50mg/kg(ip);或者苯巴比妥钠+乙醚
体离水面之前,同时猛击头部,这也被认为是人道的一种方式。 鱼类的麻醉方法 甲磺酸三卡因 水体途径 100~200mg/L 作用时间10~20min 氯丁醇 水体 20~25mg/L作用时间 10~20min 苯乙烯吡啶 水体 50~60mg/L 作用时间10~30min 乌拉坦 水体 80~95mg/L 作用时间15~30min 水合氯醛 水体 1.5~3g/L 作用时间20~40min 三氯乙醇 水体 12~25mg/L作用时间 20~40min 异戊巴比妥钠 水体 15~20mg
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