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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C. Desiccate
- 保质期:
2年
- 英文名:
DBCO-PEG5-TFP Ester-100 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
DBCO-PEG 5-TFP酯-100 mg,DBCO-PEG5-TFP Ester-100 mg,DBCO-PEG 5-TFP酯-100 mg,DBCO-PEG5-TFP Ester-100 mg
DBCO-PEG 5-TFP酯-100 mg-DBCO-PEG5-TFP Ester-100 mg
产品货号:CCT-1260-100
产品规格:100mg
DBCO-PEG 5-TFP酯是一种胺反应性标记试剂,用于修饰蛋白质、抗体和其他含胺生物聚合物。亲水性聚乙二醇(PEG)间隔臂提供了一个长而灵活的连接,最大限度地减少了与互补的含叠氮化物分子连接所涉及的空间位阻。

DBCO-PEG5-TFP Ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic polyethylene glycol (PEG) spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.The N-hydroxysuccinimide (NHS, also known as HOSu) esters of carboxylic acids are the most widely used reactive esters for modifying amine-containing peptides and proteins. NHS esters are notorious for having a short, pH-dependent half-life in aqueous media. A 2,3,5,6-tetrafluorophenol (TFP) is a different type of reactive ester that displays much better stability toward hydrolysis in aqueous media resulting in more efficiency and better reproducible labeling of biopolymers. TFP ester of carboxylic acids react with primary amines at the same rate as NHS ester forming covalent amide bond that is identical to one formed by the reaction between primary amines and NHS esters or sulfo-NHS esters.DBCO reagents are the most commonly used substrates for strain-promoted azide-alkyne click chemistry. DBCO compounds react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage.

DBCO-PEG 5-TFP酯-100 mg-DBCO-PEG5-TFP Ester-100 mg-20°C. Desiccate
点击:DBCO-PEG 5-TFP酯-100 mg-DBCO-PEG5-TFP Ester-100 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Large Scale Plasmid Preps: PEG method
Sorval tubes (max 20 mL/tube) Add an equal volume of isopropanol to each tube. Mix. Spin 15 min. at 8,000 rpm (12,000g) in a JA-10 rotor. 8. Discard supernatant. Air dry pellet. Resuspend pellets in a total of 4 mL of T.E. with 100 µg/mL
TRANSFORMATION (ONE-STEP PEG METHOD)
TRANSFORMATION (ONE-STEP PEG METHOD) Dilute 1:100 a fresh O/N culture of bacteria into prewarmed LB broth and incubate cells with shaking (225 rpm) to an OD600 of 0.3-0.4. Add an equal volume of ice-cold 2X TSS
,用G3或G4玻璃滤器过滤。(3)热聚合人IgG 将人IgC(10mg/mL)置63℃水浴加热15rain,立即置冰浴内,冷却后过Sepharose4B柱或SephacrylS-300柱,收集第一蛋白峰。所获热聚合人IgG可用考马斯亮蓝法测定蛋白含量。也可取人IgG(10mg/ml)10ml,搅拌下滴加1.8%戊二醛100uL,24h后加入0.4mol/LpH5.4,Tris-HCl缓冲液100uL终止反应,过Sepharose 4B或Sephacryl S-300柱,用0.01mol/LpH
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