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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C. Desiccate
- 保质期:
2年
- 英文名:
6-TAMRA Azide-25 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
6-塔姆拉Azide-25 mg,6-TAMRA Azide-25 mg,6-塔姆拉Azide-25 mg,6-TAMRA Azide-25 mg
6-塔姆拉Azide-25 mg-6-TAMRA Azide-25 mg
产品货号:CCT-1246-25
产品规格:25mg
6-塔姆拉叠氮化物(也称为TAMRA叠氮化物,异构体6)是红色荧光探针,与各种激发源兼容,包括汞弧、钨弧和氙弧灯、氦氖激光的544 nm线和532 nm绿色激光线。它主要用于通过铜催化点击反应(CuAAC)检测末端炔标记的生物分子。它还通过无铜的“点击化学”反应与应变环辛炔反应以形成稳定的三唑,并且不需要Cu催化剂或升高的温度。

6-TAMRA Azide (also known as TAMRA azide, isomer 6) is the red-fluorescent probe that is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CUAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.Although the mixed isomers of 5(6)-TAMRA azide is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides with TAMRA mixed isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes.6-TAMRA Azide is structurally identical to Tetramethylrhodamine (TAMRA) Azide (Tetramethylrhodamine 5-Carboxamido-(6-Azidohexanyl)), 6-isomer sold by Thermo Fisher Scientific and can be used as cost effective replacement.

6-塔姆拉Azide-25 mg-6-TAMRA Azide-25 mg-20°C. Desiccate
点击:6-塔姆拉Azide-25 mg-6-TAMRA Azide-25 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Zymolyase Dissolve in 10% glucose. Store aliquots (25 µl) at 20o C in common freezer for stock solutions Use once and dispose Comments: alternative is to dissolve in SCE, 40% gycerol, 20 mg/ml final. Can use
Oxalyticase Dissolve in 0.1 M NaCl, 0.02% Na azide, 50% glcyerole Store aliquots (1 ml) at 20o C in common freezer for stock solutions 上一篇:ALPHA FACTOR ARREST AND RELEASE 下一篇:GLASS BEADS
摘 要一、人成骨肉瘤MG-63细胞株和成人成骨细胞的培养与分化特性的观察目的:观察人成骨肉瘤MG-63细胞株和正常成人成骨细胞分化的特性。方法:在细胞培养的不同时间,用α-磷酸奈酚法测定碱性磷酸酶(ALP)活性;放射免疫法测定骨钙素(BGP)含量;半定量逆转录聚合酶链反应(RT-PCR)测I型胶原、MMP-1、TIMP-1基因mRNA表达;Van GieSon氏苦味酸酸性复红染色法染色细胞I型胶原。结果:人成骨肉瘤MG-63细胞株接种培养第7天后I型胶原基因表达较高。MMP-1表达量随时间
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