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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C. Desiccate
- 保质期:
2年
- 英文名:
Sulfo DBCO-Maleimide-500 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
Sulfo DBCO-马来酰亚胺-500 mg,Sulfo DBCO-Maleimide-500 mg,Sulfo DBCO-马来酰亚胺-500 mg,Sulfo DBCO-Maleimide-500 mg
Sulfo DBCO-马来酰亚胺-500 mg-Sulfo DBCO-Maleimide-500 mg
产品货号:CCT-1230-500
产品规格:500mg
磺基DBCO-马来酰亚胺是一种水溶性试剂,能够简单有效地将DBCO部分掺入到含半胱氨酸的肽或其他含巯基的分子上。亲水性磺化间隔臂极大地改善了DBCO衍生分子的水溶性,在许多情况下使其完全溶于水性介质。短的间隔臂对修饰的分子增加最小的质量。

Sulfo DBCO-Maleimide a water-soluble reagent that enables simple and efficient incorporation of DBCO moiety onto cysteine-containing peptides or other thiol-containing molecules. The hydrophilic sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules, in many cases making it completely soluble in aqueous media. A short spacer arm adds minimal mass to modified molecules.DBCO reagents are the most commonly used substrates for strain-promoted copper-free azide-alkyne click chemistry reactions. DBCO compounds react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage.

Sulfo DBCO-马来酰亚胺-500 mg-Sulfo DBCO-Maleimide-500 mg-20°C. Desiccate
点击:Sulfo DBCO-马来酰亚胺-500 mg-Sulfo DBCO-Maleimide-500 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Conjugation of Antibody to HRP
for no more than one hour. Re-equilibrate the column with PBS. Preparation of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add
were prepared by labeling BSA with Cy3 and Cy5 monofunctional reactive dyes (Amersham Pharmacia Biotech). Cy5-BSA-biotin was prepared by labeling Cy5-BSA with Sulfo-NHS-LC-Biotin (Pierce). Cy3-BSA-maleimide was prepared by labeling Cy3-BSA with Sulfo-GMBS
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