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- 美国
- CCT-1144-100
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C. Desiccate
- 保质期：
2年
- 英文名：
Bis-sulfone-PEG4-DBCO-100 mg
- 库存：
100
- 供应商：
艾美捷科技
- CAS号：
查看datasheet
双砜-PEG 4-DBCO-100 mg，Bis-sulfone-PEG4-DBCO-100 mg，双砜-PEG 4-DBCO-100 mg,Bis-sulfone-PEG4-DBCO-100 mg
双砜-PEG 4-DBCO-100 mg-Bis-sulfone-PEG4-DBCO-100 mg
产品货号：CCT-1144-100
产品规格：100mg
双砜-PEG 4-DBCO是一种双烷基化标记试剂，能够将DBCO部分位点特异性掺入抗体上，形成明确的缀合物。该方法涉及两个步骤：(1)二硫键还原以释放两个半胱氨酸硫醇和(2)通过经由DBCO部分共价连接的三碳桥的双烷基化重新形成二硫键。在此过程中，蛋白质的不可逆变性，破坏其三级结构或其生物活性不会发生。文献中还报道了PEG与蛋白质上的多组氨酸标签(His标签)通过双烷基化试剂的共价、位点特异性偶联(参见选定参考文献章节)。
Bis-sulfone-PEG4-DBCO is a bis-alkylating labeling reagent that enables site-specific incorporation of DBCO moiety onto antibodies to form well-defined conjugates. Conjugates prepared with bis-sulfone reagents benefits from reduced heterogeneity and enhanced stability.DBCO reagents are the most commonly used substrates for strain-promoted copper-free azide-alkyne click chemistry reactions. DBCO compounds react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage.
双砜-PEG 4-DBCO-100 mg-Bis-sulfone-PEG4-DBCO-100 mg-20°C. Desiccate
点击：双砜-PEG 4-DBCO-100 mg-Bis-sulfone-PEG4-DBCO-100 mg查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides（Biotin-Azides）试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。，请点击查看艾美捷特色产品中心。
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文献和实验RNA Purification Protocol for 100-200 mg of Animal Tissue
. Storage at -70°C. Fresh tissue can also be used. Work quickly and keep the sample on ice at all times. 2. Add 100-200 mg frozen ground tissue or fresh tissue to a 15 mL centrifuge tube containing 6 ml RCL Buffer. Homogenize the sample using 5-10
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
Steve HahnProtein Dilution Buffer 5ml20 mM Tris pH7.9 100 microliters 1M Tris 7.9150 mM KCl 0.75 ml 1 M KCl1 mM DTT 50 microliters 0.1 M DTT10% glycerol 1 ml 50% glycerol50 micrograms/ml BSA 2.5 microliters 100 mg/ml BSA3.1 ml H2OOptional: add Brij
RNAase Dissolve in 50 mM KAC pH 5.5 Heat to 100 o C for 15 minutes and cool slowly to room temperature Store at -20. (common freezer for stock solutions) 上一篇
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