Dde生物素Azide-5 mg-Dde Biotin Az

Dde生物素Azide-5 mg，Dde Biotin Azide-5 mg，Dde生物素Azide-5 mg,Dde Biotin Azide-5 mg

Dde生物素Azide-5 mg-Dde Biotin Azide-5 mg

产品货号：CCT-1136-5

产品规格：5mg

Dde Biotin Azide是一种叠氮化物激活的可切割生物素探针，可在基于亲和力的测定中有效回收亲和素结合的蛋白质复合物。 该试剂含有通过含有肼可裂解Dde部分的间隔臂与叠氮基连接的生物素部分。 在温和条件下(2%肼水溶液)，Dde liner被切割，释放生物素标签和与其结合的任何抗生物素蛋白缀合物。

Extraordinary strength of the streptavidin-biotin interaction allows for efficient capturing of even highly dilute targets; however, it makes recovery of proteins from affinity resins challenging. Conventional methods to elute biotinylated proteins from immobilized avidin include the following: (i) denaturation of streptavidin by boiling the resin in a denaturing buffer that may include high concentrations of chaotropic salts, (ii) trypsin digestion of proteins while they are bound to the resin, or (iii) elution of proteins with excess free biotin. These protocols can co-elute contaminant proteins by releasing nonspecifically bound proteins and/or naturally biotinylated proteins concurrently with labeled proteins. In addition, some of these methods can cause elution of high levels of resin-based peptides along with the proteins of interest, resulting in further sample contamination.Dde Biotin Azide probe eliminates a major limitation of the streptavidin-biotin affinity purification. This reagent contains a biotin moiety linked to an azide (N3) through a spacer arm containing a containing a hydrazine-cleavable Dde moiety. Captured biomolecules can be efficiently released under mild conditions (2% aqueous hydrazine solution), and the small molecular fragment (100.07 Da) left on the labeled protein following the cleavage. These features make the cleavable probe especially attractive for use in biomolecular labeling and proteomic studies.

Dde生物素Azide-5 mg-Dde Biotin Azide-5 mg-20°C.

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