DBCO-dPEG®羧基荧光素-5 mg-DBCO-dPEG

DBCO-dPEG®羧基荧光素-5 mg，DBCO-dPEG®₁₂-carboxyfluorescein-5 mg，DBCO-dPEG®羧基荧光素-5 mg,DBCO-dPEG®₁₂-carboxyfluorescein-5 mg

DBCO-dPEG®羧基荧光素-5 mg-DBCO-dPEG®₁₂-carboxyfluorescein-5 mg

产品货号：CCT-QBD-11812-5

产品规格：5mg

DBCO-dPEG®12-羧基荧光素，产品编号QBD-11812，允许使用应变促进叠氮化物-炔环加成(SPAAC)对肽、蛋白质和其他分子进行荧光素标记。该化合物通过中等长度(40个原子，47.6 μ m)、单分子量、离散PEG(dPEG®)间隔基将咕吨染料5(6)-羧基荧光素与流行的点击化学炔二苄基环辛炔(DBCO)结合。羧基荧光素标记具有激发波长(？ex)为498纳米(nm)，发射波长(？em)的528 nm。ε = 7.96× 104M-1cm-1。

DBCO-dPEG®12-carboxyfluorescein, product number QBD-11812, permits fluorescein labeling of peptides, proteins, and other molecules using strain-promoted azide-alkyne cycloaddition (SPAAC). This compound combines the xanthene dye, 5(6)-carboxyfluorescein, with the popular click chemistry alkyne dibenzylcyclooctyne (DBCO) through a medium-length (40 atoms, 47.6 Å), single molecular weight, discrete PEG (dPEG®) spacer. The carboxyfluorescein label has an excitation wavelength (?ex) of 498 nanometers (nm), and an emission wavelength (?em) of 528 nm. The molar absorptivity (e) is 7.96×104 M-1 cm-1 at pH 8.0 and 498 nm.Fluorescein and its derivatives are among the most popular dyes in use today for labeling proteins and cells. Unfortunately, these dyes are hydrophobic, and care must be taken to avoid over-labeling because over-labeling can result in quenching and possibly aggregation and precipitation of the conjugates through non-specific hydrophobic interactions.DBCO is an increasingly popular choice for SPAAC chemistry, also known as "copper-free click chemistry" because it avoids the use of cytotoxic copper(I) ions and allows reactions to occur in and with living cells. Although DBCO may react with free sulfhydryl groups under some conditions, it is highly selective for azides. Because azides must be introduced artificially into proteins, DBCO is considered a bioorthogonal click chemistry reagent.The introduction of a flexible dPEG® spacer between carboxyfluorescein and DBCO increases the molecule's hydrodynamic volume and imparts water solubility to DBCO-dPEG®12-carboxyfluorescein. Thus, DBCO-dPEG®12-carboxyfluorescein provides a highly selective, flexible, water-soluble way to label proteins, peptides, and cells with carboxyfluorescein using a bio-orthogonal reagent designed to simplify analysis and characterization of the conjugate.

DBCO-dPEG®羧基荧光素-5 mg-DBCO-dPEG®₁₂-carboxyfluorescein-5 mg

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