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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
2年
- 英文名:
Biotin-dPEG®₂₃-azide-1000 mg
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
查看datasheet
Biotin-dPEG®₂-azide-1000mg,Biotin-dPEG®₂₃-azide-1000 mg,Biotin-dPEG®₂-azide-1000mg,Biotin-dPEG®₂₃-azide-1000 mg
Biotin-dPEG®₂-azide-1000mg-Biotin-dPEG®₂₃-azide-1000 mg
产品货号:CCT-QBD-10787-1000
产品规格:1000mg
Biotin-dPEG®23-叠氮化物,产品编号QBD-10787,是一种在长(73个原子)单分子量离散PEG(dPEG®)链上用叠氮化物官能化的水溶性生物素化产物,用于点击化学用途。该产品能够使用金属催化(CuAAC、RuAAC)或应变促进叠氮化物-炔环加成(SPAAC)化学方法对分子进行生物素化。因此,这些产品允许新类型的生物素化构建体。由于Biotin-dPEG®23-叠氮化物是单分子量dPEG®产品,因此所得缀合物的分析得到简化。使用该产品的研究人员不必分析各种PEG链长和分子量。相反,定义明确的dPEG®产品将产生可预测的、可识别的缀合物。当将Biotin-dPEG®23-叠氮化物与经工程改造以在特定位置含有炔侧链的蛋白质或肽一起使用时,缀合位点的数量和位置都是可预测的。

Biotin-dPEG®23-azide, product number QBD-10787, is a water-soluble biotinylation product functionalized with azide for click chemistry use, on a long (73 atoms) single molecular weight, discrete PEG (dPEG®) chain. This product enables biotinylation of molecules using either metal-catalyzed (CuAAC, RuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry. Consequently, these products allow new types of biotinylated constructs.Because Biotin-dPEG®23-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®23-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, both the number and location of conjugation sites are predictable.Biotin-dPEG®23-azide has numerous uses. Through protein engineering, non-natural amino acids with alkyne side chains can be incorporated into distinct locations on proteins, thereby enabling site-specific, reproducible biotinylation of proteins. Likewise, synthetic peptides can be constructed that allow azide-alkyne click chemistry-driven biotinylation of the peptides. Also, surfaces functionalized with alkyne groups can be biotinylated using click chemistry. Furthermore, this product can be modified with other products from Vector Laboratories to create new compounds tailored to the customer’s specific needs. For example, in a 2018 study published in the journal Langmuir, researchers from The BioDesign Institute at Arizona State University used Biotin-dPEG®23-azide to construct a Y-shaped, bivalent, bis-biotin structure with a rigid core to probe the interaction of biotin and streptavidin using both atomic force microscopy (AFM) and surface plasmon resonance (SPR).As a biotinylation reagent, Biotin-dPEG®23-azide functions like other biotinylation reagents. For example, pull-down assays, affinity purification, ELISA and other plate-type assays, and supramolecular construction can employ Biotin-dPEG®23-azide successfully. Many other applications using Biotin-dPEG®23-azide are possible.

Biotin-dPEG®₂-azide-1000mg-Biotin-dPEG®₂₃-azide-1000 mg
点击:Biotin-dPEG®₂-azide-1000mg-Biotin-dPEG®₂₃-azide-1000 mg查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。,请点击查看艾美捷特色产品中心。

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文献和实验Zymolyase Dissolve in 10% glucose. Store aliquots (25 µl) at 20o C in common freezer for stock solutions Use once and dispose Comments: alternative is to dissolve in SCE, 40% gycerol, 20 mg/ml final. Can use
Oxalyticase Dissolve in 0.1 M NaCl, 0.02% Na azide, 50% glcyerole Store aliquots (1 ml) at 20o C in common freezer for stock solutions 上一篇:ALPHA FACTOR ARREST AND RELEASE 下一篇:GLASS BEADS
摘 要一、人成骨肉瘤MG-63细胞株和成人成骨细胞的培养与分化特性的观察目的:观察人成骨肉瘤MG-63细胞株和正常成人成骨细胞分化的特性。方法:在细胞培养的不同时间,用α-磷酸奈酚法测定碱性磷酸酶(ALP)活性;放射免疫法测定骨钙素(BGP)含量;半定量逆转录聚合酶链反应(RT-PCR)测I型胶原、MMP-1、TIMP-1基因mRNA表达;Van GieSon氏苦味酸酸性复红染色法染色细胞I型胶原。结果:人成骨肉瘤MG-63细胞株接种培养第7天后I型胶原基因表达较高。MMP-1表达量随时间
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