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- Click Chemistry Tools
- 美国
- CCT-QBD-10340-100
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
2年
- 英文名：
Azido-dPEG®₁₂-OH-100 mg
- 库存：
100
- 供应商：
艾美捷科技
- CAS号：
查看datasheet
Azido-dPEG®-OH-100mg，Azido-dPEG®₁₂-OH-100 mg，Azido-dPEG®-OH-100mg,Azido-dPEG®₁₂-OH-100 mg
Azido-dPEG®-OH-100mg-Azido-dPEG®₁₂-OH-100 mg
产品货号：CCT-QBD-10340-100
产品规格：100mg
Azido-dPEG®12-OH(Azido-dPEG®12-alcohol)，产品编号QBD-10340，是一种点击化学试剂，用于修饰表面和生物分子，或在超分子结构中与其他表面或生物分子交联。本品由叠氮化物和末端羟基组成，中间由中等长度(37个原子)、单分子量、离散长度聚乙二醇(dPEG®)链隔开。末端叠氮化物在铜(I)催化、铼催化和应变促进的叠氮化物-炔环加成反应(分别为CuAAC、RuAAC和SPAAC)中反应。如果保持未改性，则醇基团增加其所缀合的分子和表面的水溶性和流体动力学体积。用反应性基团如氯化物、甲苯磺酸盐或甲磺酸盐修饰醇允许Azido-dPEG®12-OH通过随后的化学操作充当交联剂。
Azido-dPEG®12-OH (Azido-dPEG®12-alcohol), product number QBD-10340, is a click chemistry reagent used to modify surfaces and biomolecules or to crosslink with other surfaces or biomolecules in supramolecular construction. This product consists of an azide and a terminal hydroxy group separated by a medium-length (37 atoms), single molecular weight, discrete-length polyethylene glycol (dPEG®) chain. The terminal azide reacts in copper(I)-catalyzed, ruthenium-catalyzed, and strain-promoted azide-alkyne cycloaddition reactions (CuAAC, RuAAC, and SPAAC, respectively). If left unmodified, the alcohol group increases the water solubility and hydrodynamic volume of molecules and surfaces to which it is conjugated. Modifying the alcohol with a reactive group such as chloride, tosylate, or mesylate allows Azido-dPEG®12-OH to function as a crosslinker through subsequent chemical manipulations.As a click chemistry reagent, azido-dPEG®12-OH can participate in CuAAC, RuAAC, and SPAAC reactions. Because Vector Laboratories' dPEG® products are amphiphilic, these reactions can occur almost equally well in water or organic solvents. Thus, azido-dPEG®12-OH can be used in conjugations with biomolecules where organic solvents may damage the conjugate target.The primary alcohol group on the opposite end of the linker can be used as a neutrally charged functional group. In this function, the alcohol enhances the water solubility of the conjugate molecule. Alternatively, the alcohol group can be functionalized with reactive groups and used to modify the conjugate further.
Azido-dPEG®-OH-100mg-Azido-dPEG®₁₂-OH-100 mg
点击：Azido-dPEG®-OH-100mg-Azido-dPEG®₁₂-OH-100 mg查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides（Biotin-Azides）试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。，请点击查看艾美捷特色产品中心。
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文献和实验Zymolyase Dissolve in 10% glucose. Store aliquots (25 µl) at 20o C in common freezer for stock solutions Use once and dispose Comments: alternative is to dissolve in SCE, 40% gycerol, 20 mg/ml final. Can use
Oxalyticase Dissolve in 0.1 M NaCl, 0.02% Na azide, 50% glcyerole Store aliquots (1 ml) at 20o C in common freezer for stock solutions 上一篇：ALPHA FACTOR ARREST AND RELEASE 下一篇：GLASS BEADS
摘 要一、人成骨肉瘤MG-63细胞株和成人成骨细胞的培养与分化特性的观察目的：观察人成骨肉瘤MG-63细胞株和正常成人成骨细胞分化的特性。方法：在细胞培养的不同时间，用α-磷酸奈酚法测定碱性磷酸酶（ALP）活性；放射免疫法测定骨钙素（BGP）含量；半定量逆转录聚合酶链反应（RT-PCR）测I型胶原、MMP-1、TIMP-1基因mRNA表达；Van GieSon氏苦味酸酸性复红染色法染色细胞I型胶原。结果：人成骨肉瘤MG-63细胞株接种培养第7天后I型胶原基因表达较高。MMP-1表达量随时间
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