生物素-dPEG ® β-酰肼-50 mg-Biotin-d

生物素-dPEG ® β-酰肼-50 mg，Biotin-dPEG®₄-hydrazide-50 mg，生物素-dPEG ® β-酰肼-50 mg,Biotin-dPEG®₄-hydrazide-50 mg

生物素-dPEG ® β-酰肼-50 mg-Biotin-dPEG®₄-hydrazide-50 mg

产品货号：CCT-QBD-10219-50

产品规格：50mg

Biotin-dPEG®4-酰肼，产品编号QBD-10219，是一种亲水性生物素化试剂，设计用于用生物素标记糖蛋白的碳水化合物涂层。两亲间隔基为水溶性差的生物素提供了优异的水溶性。此外，dPEG®4间隔基比生物素-LC-酰肼中使用的疏水性氨基己酸(LC)间隔基更长，因此它提供了对亲和素和链霉亲和素的生物素结合口袋的更好的接近。此外，dPEG®4间隔基在许多应用中减少或消除非特异性结合。

Biotin-dPEG®4-hydrazide, product number QBD-10219, is a hydrophilic biotinylation reagent designed for labeling the carbohydrate coats of glycoproteins with biotin. The amphiphilic spacer provides excellent water solubility for poorly water-soluble biotin. Moreover, the dPEG®4 spacer is longer than the hydrophobic aminocaproic acid (LC) spacer used in biotin-LC-hydrazide, so it provides somewhat better access to the biotin binding pockets of avidin and streptavidin. Additionally, the dPEG®4 spacer reduces or eliminates non-specific binding in many applications.Biotin-dPEG®4-hydrazide, QBD-10219, is a carbonyl-reactive compound that forms semi-permanent hydrazone bonds with aldehydes, ketones, and carboxylic acid groups. Although there are numerous natural small molecules containing aldehydes and ketones, macromolecules of biological importance (peptides, proteins, and nucleic acids) rarely contain them. However, glycosylated proteins such as antibodies frequently contain carbohydrate residues known as reducing sugars that can be oxidized with sodium periodate, neuraminidase, or galactose oxidase to form aldehydes.The aldehydes thus formed then can be reacted with Biotin-dPEG®4-hydrazide at pH 5 – 7, labeling the protein with biotin through the formation of a Schiff base. Aniline or para-phenylenediamine catalyzes the reaction, forming the Schiff base quickly. For additional bond stability, sodium cyanoborohydride can be used to reduce the Schiff base to a secondary amine. Via carbodiimide chemistry (for example, 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide, also known as EDC), Biotin-dPEG®4-hydrazide reacts with carboxylic acid groups forming a stable bond.The amphiphilic dPEG®4 spacer in Biotin-dPEG®4-hydrazide provides the ability to use Biotin-dPEG®4-hydrazide directly in water or aqueous buffer, making it more biocompatible than LC-biotin. Moreover, QBD-10219 will not trigger protein or peptide aggregation or cause non-specific binding due to hydrophobicity issues from the linker, both of which can occur with traditional biotin linkers like LC-biotin.Biotin-dPEG®4-hydrazide is highly useful for installing a biotin label onto glycoproteins. It is also used in cell labeling to label glycoproteins on cell surfaces. Biotinylated glycoproteins can be identified using streptavidin-horseradish peroxidase (HRP) conjugates, because of the high affinity between biotin and streptavidin. Biotinylated secondary antibodies are used frequently with avidin or streptavidin in plate-based assays such as ELISA and western blots. This reagent, coupled with avidin that has been modified to have reduced biotin-binding affinity, is for affinity purification of molecules.

生物素-dPEG ® β-酰肼-50 mg-Biotin-dPEG®₄-hydrazide-50 mg

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