Agilent安捷伦 G2855-28505CFT Ferr

RecoveringDNAfromagarosegels

is purification by placing the gel slice within a microfuge tube containing a membrane with a small pore size, such as a Costar Spin-X microfuge tube fitted with a 0.22 um filter [12] . Alternatively, gel slices composed of low melting point (LMP) agarose

Recovering DNA from agarose gels

a membrane with a small pore size, such as a Costar Spin-X microfuge tube fitted with a 0.22 um filter [12]. Alternatively, gel slices composed of low melting point (LMP) agarose can be placed in a microfuge at 70 degrees C until the gel has melted

张峰手稿：基因工程技术之CRISPR-Cas9

polymerase (Agilent). Otherhigh-fi delity polymerases, such as Kapa HiFi (Kapa Biosystems)can also be used for PCR in this protocol. 7. 10× Taq PCR buffer. 8. Novex Hi-Density TBE sample buffer (Life Technologies). 9. 10,000× SYBR Gold nucleic acid