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文献和实验is approximately 2.7:1; but a 2:1 ratio has long been considered the benchmark for intact RNA. While crisp 28S and 18S rRNA bands are indicative of intact RNA, it is less clear how these long-lived and abundant molecules actually reflect the quality of the underlying mRNA
Embryo Dissection and Micromanipulation Tools
against the microscope stage. Label the forceps for different uses: good forceps should be reserved for fine work, and old battered forceps (which can be bent and filed back to a blunt end) should be designated for all noncritical purposes. Needle-nose pliers
will go a long way towards minimizing RNase contamination problems. Surfaces Laboratory surfaces, such as benchtops, centrifuges and electrophoresis equipment, should be assumed to be contaminated with RNases, since they are usually exposed
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