Agilent安捷伦 7550701CVersaPlate

Determining In Vivo Phosphorylation Sites Using Mass Spectrometry

1‐ml sample loop High‐flow‐rate HPLC (offline SCX fractionation): e.g., Agilent 1200, Michrom Paradigm MG4 (Bruker), Shimadzu Prominence

Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT)

capacity and a loading volume of up to 800 µl. Fused-silica capillary, 100 µm I.D. x 365 µm O.D. (Agilent Technologies or Polymicro Technologies) Fused-silica capillary (size and length depend on the desired split flow rate, see Step 10) Gold

Identification of Proteins in Complex Mixtures Using Liquid Chromatography and Mass Spectrometry

Cyanogen bromide (e.g., Aldrich), for insoluble fractions or membrane proteins only 96% (v/v) formic acid (e.g., Aldrich), for insoluble fractions or membrane proteins