Agilent安捷伦 14102042HF Bond Elu

ChIP-Chip E. coli

mg/ml Proteinase K in TBS (store up to 1 year at -20 C) 1 ml 100mM PMSF or 0.5 ml 250 mM Pefabloc   Final concentration For 50ml Lysis Buffer 10mM Tris (pH 8.0) 500μL 1

Proteasome Preparation from Human Brain

with water. Make up the Homogenising Buffer (3ml/g tissue) in the following way:- Tris 500mM = 30.3g/500ml. Adjust to pH7.5 with HCl. ATP 100mM = 0.551g/10ml. Adjust to pH7.5 with NaOH (can be stored at -20°C). MgCl2 50mM = 1.02g/100ml. DTT

正相硅胶/选择洗脱―气相色谱法、液相色谱―质谱法检测

公司);T�18basic均质器(德国IKA公司);低速离心机(德国Sigma公司);旋转蒸发仪(德国Heidolph公司);DC�12氮吹仪(上海安谱公司);标准品(德国DR公司);PSA粉末、LC�Si固相萃取小柱500 mg/6 mL(美国Supelco公司);所用试剂均为分析纯或色谱纯;实验用水为三重过滤去离子水。 　　2.2 实验方法 　　2.2.1 样品提取 (1)新鲜样品 称取匀浆样品2 g至50 mL离心管中，加入乙酸乙酯20 mL，无水Na2SO4 8 g，用均质