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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
Inc.) or QiaFilter™ (Qiagen Inc.) plate positioned over a 96 deep-well collection block inside a vacuum manifold. Transfer 600 ul at a time, collecting the top half (with flocculent ppt.) first. Vacuum filter using approximately 300-500 mm Hg until filtration
% ethanol. Incubate at least 2 hours at -20degC, centrifuge at 10,000 rpm for 45 minutes in the RC5-B using the SS-34 rotor. Gently decant the supernatant, add 80% ethanol, centrifuge as before, decant, and dry the DNA pellet in a vacuum oven. 12
Staining C. elegans for ß-galactosidase
as possible using a pipetteman. 3. Cap the tubes and freeze in liquid nitrogen. Open the caps and stick in a vacuum jar or speed vac for about 45 minutes to lyophilize. 4. Add ~250 ul cold acetone, and let sit in the freezer 3 min. Remove
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