- ￥10659
- BPS Bioscience
- 美国
- 101355
- 2026年02月03日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
Protein
- 供应商：
盛祺兆生物
- 规格：
4 x 50 µg
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文献和实验Small scale His-Tag fusion protein purification under denaturative conditions
Small scale His-Tag fusion protein purification under denaturative conditions Introduction High levels of expression of recombinant proteins in a bacterial system can lead to the formation of insoluble aggregates
Methods2.1. Material and reagentsDuolbecco’s phosphate buffered saline (PBS), Medium 199 with Earle’s salts and 25mM HEPES, collagenase Type II (from Clostridum histolyticum), antibiotic/antimyotic solutuion (containing 10,000U penicillin, 10,000µg
Characterization of Histaminergic Receptors
phosphate‐buffered saline (DPBS; calcium‐ and magnesium‐free; see recipe ), ice‐cold Na+ /K+ assay buffer (see recipe ), 25°C 20 µM promethazine (Research Biochemicals) in 50 mM Na
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