GPC3, Avi-His-Tagged, Biotin-L

Preparation and purification of biotin-labeled probe for ISH

is collected in the 1.5 ml tube The final volume of probe should be close to 55 µl. Usually, 5 µl of such a biotin-labeled probe (1 µg/55 µl = 18 ng/µl) is enough for one slide, and the probe can be used to process 100 slides. The size of the probe

CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA

  DAY 1: 1) Reprecipitate DNA's Add the following DNA's to a 1.5 ml centrifuge tube, mixing with pipet: 20 ug Cot-1 DNA (~20ul) ~200 ng Biotin labeled DNA (~10ul) ~200 ng Digoxigenin labeled DNA (~10ul) Add 1/10th

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM