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- 详细信息
- 文献和实验
- 技术资料
- 库存:
Protein
- 供应商:
盛祺兆生物
- 规格:
5 µg
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文献和实验Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors
The molecular cloning of the cDNA sequences encoding most G-protein-coupled receptors, including those from humans, allows their study in a variety of recombinant systems. In this respect, transfected mammalian cell lines constitute
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
have a promoter upstream of the MCS such that the insert gets transcribed. If the promoter is mammalian (e.g., the CMV promoter), the transcription will take place only in mammalian systems (like mammalian cell culture), but not in the bacteria. Plasmids
DETECTION OF RECOMBINANT PROTEINS BY ELISA
which we use for bacterially expressed recombinant proteins). Controls will include no antigen, and extracts from bacteria transformed with the appropriate expression vector which lack the insert. Add 50µl per well of the appropriate solution in coating buffer
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