- ￥2485.44
- BPS Bioscience
- 美国
- 50351
- 2025年11月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
Substrate
- 供应商：
盛祺兆生物
- 规格：
500 µl
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文献和实验complexes by pulse centrifugation (15 seconds at 3,000 RPM). Wash the bead/complexes 3 times in 500µl ice-cold TBS-mod buffer(comment 4); ;To elute the putative ChIP-Flag complexes (by competition with 3x Flag-peptide) add 100µl of TBS-mod buffer+3µl (5µg/µl
Peptide Pull-Down (PPD) Assay for Identification and Characterization of Histone PTM Effectors
of biotinylated peptide in 400 µL PBS. (See note 2) Wash 400 µL avidin beads at least 3 times with 1 mL PBS + 0.1% Triton X-100, spin and remove supernatant. (See note 3) Apply the 400 µL re-suspended peptide to the 400 µL washed beads
seconds at 3,000 RPM). Wash the bead/complexes 3 times in 500µl ice-cold TBS-mod buffer (comment 4);To elute the putative ChIP-Flag complexes (by competition with 3x Flag-peptide) add 100µl of TBS-mod buffer+3µl (5µg/µl 3X Flag Peptide) to each pelleted
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