- ￥9945
- BPS Bioscience
- 美国
- 82185-1
- 2025年09月04日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
Assay Kit Component
- 供应商：
盛祺兆生物
- 规格：
40 µl
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文献和实验Purification of recombinant sBRF M166L
by resuspension and centrifugation. Add the equilibrated beads to the clarified extract and place on a room temp. shaker platform for 45 min. Centrifuge the bead-extract mix in the GSA rotor for 5 min at 5K RPM and carefully remove the supernatant (the pellet
Purification of recombinant sBRF M166L
the equilibrated beads to the clarified extract and place on a room temp.shaker platform for 45 min. Centrifuge the bead-extract mix in the GSA rotor for 5 min at 5K RPM and carefully remove the supernatant (the pellet of beads will not be very firm
and then centrifuge at 14,000 RPM for 3 minutes. Save 20µl of the elutes as (Flag-Input). Next, take the remaining ~80µl of elute to clean tubes containing 40µl of EZVIEW™ Red anti-Flag M2 affinity gel beads (Sigma™) which have been pre-treated with a wash in 250µl
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