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- 2025年12月05日
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产品活性：Tubulin polymerization/V-ATPase-IN-1 (compound F10) 是微管蛋白聚合/V-ATPase (Tubulin polymerization/V-ATPase) 抑制剂。Tubulin polymerization/V-ATPase-IN-1 对四种人类癌细胞表现出强大的抗增殖活性，并通过抑制微管蛋白和 V-ATPase 发挥抗增殖活性。Tubulin polymerization/V-ATPase-IN-1 诱导细胞凋亡 (apoptosis) 和免疫原性死亡，并抑制 T 淋巴细胞浸润增强的 RM-1 同种移植物模型的肿瘤生长。
产品来源： www.medchemexpress.cn/tubulin-polymerization-v-atpase-in-1.html
研究领域：Cytoskeleton | Cell Cycle/DNA Damage | Membrane Transporter/Ion Channel | Apoptosis
作用靶点：Microtubule/Tubulin | Proton Pump | Apoptosis
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文献和实验SDS Gel Electrophoresis of Tubulin\MAPs
2% SDS 10% glycerol 5% -mercaptoethanol 0.001 % bromophenol blue TEMED 10% (w/v) Ammonium persulphate 50% and 7% (w/v) TCA (trichloroacetic acid) 0.1% (w/v) Coomassie brilliant blue in 50
Large Scale Tubulin Preparation
Tubulin is purified from bovine/porcine brain by two cycles of polymerization/depolymerization followed by removal of copurifying proteins on a phosphocellulose (PC) column. The procedure described here is for a large scale prep (10 cow brains
Tubulin Polymerization with GTP/GMPCPP/Taxol
all at once it will cause tubulin precipitation! If polymerizing 2 mg/ml tubulin, use 2 µM, 20 µM and 200 µM steps OR II . Add 10%(v/v) DMSO and incubate at 37¡C for 20'-30' Pellet and resuspend microtubules as described above For DMSO polymerization
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