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Please store the product under the recommended conditions in the Certificate of Analysis.
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货期:询盘
- 供应商:
MedChemExpress LLC
- CAS号:
161877-70-9
- 规格:
询盘
Dabcyl-YVADAPV-EDANS
产品活性:Dabcyl YVADAPV EDANS 是一种荧光白细胞介素-1β 转化酶 (interleukin-1β converting enzyme, ICE) 底物,用于检测 ICE 样蛋白酶活性。Dabcyl YVADAPV EDANS 检测荧光的激发波长为 360 nm,发射波长为 480 nm。ICE 样蛋白酶是 K+ 剥夺诱导小脑颗粒神经元凋亡 (apoptosis) 的关键介质。
产品来源: www.medchemexpress.cn/dabcyl-yvadapv-edans.html
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: ICE activity assay methods:(1) Cerebellar granule neurons were seeded in 24-well plates at a density of 2.1*105 cells/cm2.(2) After the switch to medium containing low (5 mM) or high (25 mM) concentrations of KCl at DIV 8, cerebellar granule neurons were washed with Locke’s buffer and made permeable by 0.03% Digitonin (HY-N4000).(3) After 10 min, DABCYL-YVADAPV-EDANS (20 μM) was added. (4) Fluorescence was determined in 10 min intervals for 1 h using 360 nm excitation and 480 nm emission wavelengths in the 24-well plates.(5) The fluorometric intensity peaked at 20 min. This time point was used for statistical analysis.
相关产品:MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | CFDA-SE | FITC-Dextran (MW 4000) | Dihydroethidium | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | CY5 | TMRE | Fluo-4 AM | Indocyanine green | PKH 26 | Acridine Orange hydrochloride | DiD perchlorate | FM4-64 | BODIPY 581/591 C11 | CY5-SE | DiO
产品活性:Dabcyl YVADAPV EDANS 是一种荧光白细胞介素-1β 转化酶 (interleukin-1β converting enzyme, ICE) 底物,用于检测 ICE 样蛋白酶活性。Dabcyl YVADAPV EDANS 检测荧光的激发波长为 360 nm,发射波长为 480 nm。ICE 样蛋白酶是 K+ 剥夺诱导小脑颗粒神经元凋亡 (apoptosis) 的关键介质。
产品来源: www.medchemexpress.cn/dabcyl-yvadapv-edans.html
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: ICE activity assay methods:(1) Cerebellar granule neurons were seeded in 24-well plates at a density of 2.1*105 cells/cm2.(2) After the switch to medium containing low (5 mM) or high (25 mM) concentrations of KCl at DIV 8, cerebellar granule neurons were washed with Locke’s buffer and made permeable by 0.03% Digitonin (HY-N4000).(3) After 10 min, DABCYL-YVADAPV-EDANS (20 μM) was added. (4) Fluorescence was determined in 10 min intervals for 1 h using 360 nm excitation and 480 nm emission wavelengths in the 24-well plates.(5) The fluorometric intensity peaked at 20 min. This time point was used for statistical analysis.
相关产品:MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | CFDA-SE | FITC-Dextran (MW 4000) | Dihydroethidium | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | CY5 | TMRE | Fluo-4 AM | Indocyanine green | PKH 26 | Acridine Orange hydrochloride | DiD perchlorate | FM4-64 | BODIPY 581/591 C11 | CY5-SE | DiO
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Dabcyl-YVADAPV-EDANS,161877-70-9
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