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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
NA
- 保质期:
NA
- 英文名:
RPF Growth Medium: All-in-one ready-to-use, for Rat Perineurial Fibroblasts.
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
R827-500
- 规格:
500ml
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文献和实验primary fibroblasts (˜ cell number 1 x 106 ) use: A. 25 ml rat tail collagen (approx conc 2mg/ml) B. 3 ml 10xMEM C. 0.22 M NaOH: first, add 2ml, then drop-wise whilst stirring, until the collagen turns
Biological coating procedures for glass coverslips
temperature. Aspirate solution and wash coverslips 3 times with media or PBS. Immediately add cell suspension or growth media. FIBRONECTIN Fibronectin is an extracellular matrix constituent use for the culture of endothelial cells
% RPMI 1640 10% Heat-inactivated horse serum 5% Fetal bovine serum Glutamine Differentiating Medium 92.5% RPMI 1640 5% Heat-inactivated horse serum 2.5% Fetal bovine serum 2 nM Nerve growth factor (Boehringer Mannheim #1058 231
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