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Protein G Bestarose 4FF常用于分离纯化来自细胞培养物中的抗体或者抗体片段，以及从各种种属的血清中纯化抗体。
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文献和实验[1] Wang Z, Shi W, Wu T, Peng T, Wang X, Liu S, Yang Z, Wang J, Li PL, Tian R, Hong Y, Yang H, Bai L, Hu Y, Cheng X, Li H, Zhang XJ, She ZG. A high-throughput drug screening identifies luteolin as a therapeutic candidate for pathological cardiac hypertrophy and heart failure. Front Cardiovasc Med. 2023 Mar 14;10:1130635. doi: 10.3389/fcvm.2023.1130635. PMID: 36998980; PMCID: PMC10043402.
[2] Zhang XJ, Li HL, Deng DY, Ji C, Yao XD, Liu JX. Functional and proteomic comparison of different techniques to produce equine anti-tetanus immunoglobulin F(ab')2 fragments. J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Aug 15;1092:29-39. doi: 10.1016/j.jchromb.2018.05.041. Epub 2018 May 29. PMID: 29883887.
[3] Han J, Pan Y, Gu Y, Xu X, Zhao R, Sha J, Zhang R, Gu J, Ren S. Profiling of IgG N-glycome during mouse aging: Fucosylated diantennary glycans containing one Neu5Gc-linked LacNAc are associated with age. J Proteomics. 2020 Oct 30;229:103966. doi: 10.1016/j.jprot.2020.103966. Epub 2020 Sep 3. PMID: 32891889.
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Protein A, derived from the cell wall of Staphylococcus aureus , and protein G, derived from the cell wall of certain strains of b -hemolytic Streptococcci , binds the Fc region
G Protein Abnormalities in Schizophrenia
of a certain receptor subtypes does not always mean that the downstream partners of the receptor (G proteins, effectors, protein kinases, phosphatases) are normal.
Protein G Purification of Antibodies
1. Reagent and Materials(1) Hi-Trap Protein G Column (Pharmacia Biotech #17-0404-01)(2) 20 mM Sodium Phosphate Buffer, pH 7.01.084 g NaH2PO4, anhydrous3.273 g Na2HPO4.7H2Oq.s. to 1 liter with di-H2O(3) 0.1M Glycine, pH 2.83.75 g Glycine1.4 ml HCl
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