大鼠内皮型一氧/化氮合成酶(NOS3/eNOS)ELISA试

英文名	Rat NOS3/eNOS ELISA Kit
别名	ECNOS; NOS 鈪#; E-NOS
相关类别	ELISA	储存	冷藏（2-8℃）

 

应用

用于大鼠血清、血浆或其他相关生物液体中内皮型一氧/化氮合成酶的测定。
For the determination of Rat NOS3/eNOS(Nitric Oxide Synthase 3, Endothelial) concentrations in serum, plasma and other related biological fluids.

 

属性

工作原理	本试剂盒采用的是双抗体夹心法酶联免疫吸附检测技术（ELISA）。测定样品中大鼠内皮型一氧/化氮合成酶水平。向预先包被了抗大鼠内皮型一氧/化氮合成酶抗体的酶标孔中，加入标准品和样本，温育后，加入生物素标记的抗内皮型一氧/化氮合成酶抗体。再与HRP标记的链霉亲和素结合，形成免疫复合物，再经过温育和洗涤，去除未结合的酶，然后加入显色底物TMB，产生蓝色，并在酸的作用下转化成最终的黄色。最后，在450 nm处测定反应孔样品吸光度（OD）值，样本中的大鼠内皮型一氧/化氮合成酶浓度与OD值成正比，通过绘制标准曲线计算出样本中大鼠内皮型一氧/化氮合成酶的浓度。
Purpose	This assay employs the Sandwich enzyme immunoassay technique. Determine the level of Rat NOS3/eNOS(Nitric Oxide Synthase 3, Endothelial) in the sample. Add standard and sample to the microplate that have been pre-coated with Anti-Rat NOS3/eNOS(Nitric Oxide Synthase 3, Endothelial) antibody. After incubation, add Biotin-conjugated anti-Rat NOS3/eNOS(Nitric Oxide Synthase 3, Endothelial) antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Rat NOS3/eNOS(Nitric Oxide Synthase 3, Endothelial) in the sample was proportional to the OD value. The concentration of Rat NOS3/eNOS(Nitric Oxide Synthase 3, Endothelial) in the sample can be calculated by drawing a standard curve.
灵敏度	9.38 pg/mL
Sensitivity	9.38 pg/mL
检测范围	15.63-1000 pg/mL
Examination range	15.63-1000 pg/mL
特异性	可检测样本中的大鼠内皮型一氧/化氮合成酶，且与其类似物无明显交叉反应。
Specificity	This kit recognizes Rat NOS3/eNOS(Nitric Oxide Synthase 3, Endothelial) in samples. No significant cross-reactivity or interference between Rat NOS3/eNOS(Nitric Oxide Synthase 3, Endothelial) and analogues was ob/served.
重复性	三种不同浓度的样本在同一块板子上分别检测20次，以及在不同的板子上分别检测20次，板内和板间变异系数均小于10%。
Repeatability	Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%.
回收率	分别往稀释不同倍数的5个样本中加入已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。
Recovery	A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate.